F4/80 anticorps

N° du produit ABIN488117

Détail du produit anti-F4/80 anticorps

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp F4/80 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Immunoelectron Microscopy (IEM)

Specificité: This antibody recognises the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EMR1. Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Immunogène: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Clone: Cl:A3-1

Isotype: IgG2b

Information d'application

Indications d'application: RIA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. Thisproduct requires pre-treatment of paraffin sections (Proteinase K is recommended fortissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixedfor more than 24 hours).

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-F4/80 (ABIN488117)

Schindewolffs, Breves, Buettner, Hadamitzky, Pabst: "VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment." dans: Immunity, inflammation and disease, Vol. 2, Issue 3, pp. 152-61, (2014) (PubMed).

Cuzi?, Bosnar, Kramari?, Ferenci?, Markovi?, Glojnari?, Erakovi? Haber: "Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette smoke-induced epithelial injury along alveolar ducts." dans: Toxicologic pathology, Vol. 40, Issue 8, pp. 1169-87, (2012) (PubMed).

Nakao, Zandi, Faez, Kohno, Hafezi-Moghadam: "Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 808-17, (2012) (PubMed).

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Hakroush, Moeller, Theilig, Kaissling, Sijmonsma, Jugold, Akeson, Traykova-Brauch, Hosser, Hähnel, Gröne, Koesters, Kriz: "Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease." dans: The American journal of pathology, Vol. 175, Issue 5, pp. 1883-95, (2010) (PubMed).

Spyridonidou, Fousteris, Antonia, Chatzianastasiou, Papapetropoulos, Nikolaropoulos: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." dans: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).

Kimura, Miyashita, Suzuki, Kobayashi, Watanabe, Sonoda, Ohta, Fujiwara, Shimosegawa, Sato: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." dans: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).

Kakehashi, Nishioku, Tsukuba, Kadowaki, Nakamura, Yamamoto: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).

Anghelina, Krishnan, Moldovan, Moldovan: "Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles." dans: The American journal of pathology, Vol. 168, Issue 2, pp. 529-41, (2006) (PubMed).

Détails sur F4/80

Antigène: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Autre désignation: Macrophage F4/80 Antigen (EMR1 Produits)

Synonymes: anticorps EMR1, anticorps TM7LN3, anticorps DD7A5-7, anticorps EGF-TM7, anticorps F4/80, anticorps Gpf480, anticorps Ly71, anticorps adhesion G protein-coupled receptor E1, anticorps ADGRE1, anticorps LOC100424335, anticorps Adgre1

Sujet: F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages. Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

ID gène: 13733

NCBI Accession: NP_034260

UniProt: Q61549