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NEDD8 anticorps (Middle Region)

NEDD8 Reactivité: Humain, Souris, Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN4886679
  • Antigène Voir toutes NEDD8 Anticorps
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Épitope
    • 15
    • 13
    • 8
    • 3
    • 3
    • 1
    • 1
    AA 20-60, Middle Region
    Reactivité
    • 66
    • 28
    • 24
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 63
    • 4
    • 4
    Lapin
    Clonalité
    • 59
    • 12
    Polyclonal
    Conjugué
    • 30
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp NEDD8 est non-conjugé
    Application
    • 38
    • 27
    • 27
    • 13
    • 13
    • 12
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Fonction
    Rabbit IgG polyclonal antibody for NEDD8(NEDD8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Séquence
    TDKVERIKER VEEKEGIPPQ QQRLIYSGKQ MNDEKTAADY K
    Réactivité croisée (Details)
    No cross reactivity with other proteins.
    Attributs du produit
    Rabbit IgG polyclonal antibody for NEDD8(NEDD8) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
    Gene Name: neural precursor cell expressed, developmentally down-regulated 8
    Protein Name: NEDD8
    Purification
    Immunogen affinity purified.
    Immunogène
    A synthetic peptide corresponding to a sequence in the middle region of human NEDD8 (20-60aa TDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYK), identical to the related mouse and rat sequences.
    Isotype
    IgG
    Top Product
    Discover our top product NEDD8 Anticorps primaire
  • Indications d'application
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Commentaires

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Concentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freezing and thawing.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
  • Antigène
    NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
    Autre désignation
    NEDD8 (NEDD8 Produits)
    Synonymes
    anticorps NEDD-8, anticorps Rub1, anticorps CG10679, anticorps Dmel\\CG10679, anticorps NEDD8, anticorps dNEDD8, anticorps nedd8, anticorps ubiquitin, anticorps si:zc14a17.5, anticorps GB17785, anticorps DDBDRAFT_0218116, anticorps DDBDRAFT_0238041, anticorps DDB_0218116, anticorps DDB_0238041, anticorps nedd8.S, anticorps neural precursor cell expressed, developmentally down-regulated 8, anticorps neural precursor cell expressed, developmentally down-regulated gene 8, anticorps CG10679 gene product from transcript CG10679-RB, anticorps Nedd8, anticorps ubiquitin-like protein Nedd8, anticorps neural precursor cell expressed, developmentally down-regulated 8 L homeolog, anticorps NEDD8, anticorps Nedd8, anticorps nedd8, anticorps nedd8.L
    Sujet
    NEDD8 is a protein that in humans is encoded by the NEDD8 gene. Human NEDD8 shares 60 % amino acid sequence identity to ubiquitin. The most substrates of NEDD8 modification are the Cullin subunits of Cullin-based E3 ubiquitin ligases, which are active only when neddylated. Their NEDDylation is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.

    Synonyms: NED8 | NEDD 8 | NEDD-8 | Nedd8 | Neddylin | Rub1 | Q15843
    ID gène
    4738
    UniProt
    Q15843
    Pathways
    Ubiquitin Proteasome Pathway
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