CELF1 anticorps

N° du produit ABIN498349

Détail du produit anti-CELF1 anticorps

Antigène: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CELF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of CUGBP1/2 protein. (region surrounding Lys95)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: CUGBP2

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur CELF1

Antigène: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Autre désignation: CUGBP1 (CELF1 Produits)

Synonymes: anticorps CUGBP1, anticorps BRUNOL2, anticorps CUG-BP, anticorps CUGBP, anticorps EDEN-BP, anticorps NAB50, anticorps NAPOR, anticorps hNab50, anticorps 1600010O03Rik, anticorps AA407467, anticorps Brunol2, anticorps CUG-BP1, anticorps Cugbp1, anticorps D2Wsu101e, anticorps HNAB50, anticorps Bruno-like, anticorps brul, anticorps cb920, anticorps cugbp1, anticorps brunol2, anticorps cug-bp, anticorps cug-bp1, anticorps cugbp, anticorps cugbp1-a, anticorps eden-bp, anticorps edenbp, anticorps nab50, anticorps CUGBP Elav-like family member 1, anticorps CUGBP, Elav-like family member 1, anticorps cugbp, Elav-like family member 1, anticorps CUGBP Elav-like family member 1 L homeolog, anticorps CELF1, anticorps Celf1, anticorps celf1, anticorps celf1.L

Sujet: Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPbeta transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPbeta mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawal.Synonyms: 50 kDa nuclear polyadenylated RNA-binding protein, BRUNOL2, Bruno-like protein 2, CELF-1, CELF1, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, CUGBP, Deadenylation factor CUG-BP, Embryo deadenylation element-binding protein homolog, NAB50, RNA-binding protein BRUNOL-2

Poids moléculaire: approx. 52 kDa

ID gène: 10658

NCBI Accession: NP_001020767

UniProt: Q92879

Pathways: Ribonucleoprotein Complex Subunit Organization