STAT4 anticorps (Internal Region)

N° du produit ABIN499124

Détail du produit anti-STAT4 anticorps

Antigène: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Épitope: AA 613-627, Internal Region

Reactivité: Humain, Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT4 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: C-DHSESGEVRF HSVEP

Specificité: Recognizes STAT4 (aa 613-627).

Réactivité croisée (Details): Species reactivity (expected):Canine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region (aa 613-627) of the protein sequence according to Human STAT4 (NP_003142.1.) Genename: STAT4

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/32000. Western blot: 0.2-0.5 μg/mL. This antibody detects a band of approx. 85 kDa in transfectedHEK293 transiently expressing full-length Human STAT4 (MYC and DYKDDDDK tagged). No bands were observed in mock-transfected HEK293 and the same band was observedusing anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur STAT4

Antigène: STAT4 (Signal Transducer and Activator of Transcription 4 (STAT4))

Autre désignation: STAT4 (STAT4 Produits)

Synonymes: anticorps SLEB11, anticorps signal transducer and activator of transcription 4, anticorps STAT4, anticorps stat4, anticorps Stat4

Sujet: The STAT 4 protein is a transcription factor activated by hormone and cytokines. STAT 4 is most similar to STAT 1 (52 % ) and STAT 3 (47 %). Functionally STAT 4 is similar to other STAT family members in that it can be tyrosine phosphorylated by JAK 1 or JAK 2. STAT 4 forms homodimers and heterodimers with related STAT family members. Tyrosine phosphorylated STAT 4 can bind the interferon g activated site (GAS), and serine phosphorylation is also required for maximal transcriptional activity. STAT 4 expression is restricted to the thymus, spleen, and testis.Synonyms: Signal transducer and activator of transcription 4

ID gène: 6775

NCBI Accession: NP_003142

UniProt: Q14765

Pathways: Signalistation JAK/STAT