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ISR-alpha anticorps (AA 51-150) (Alexa Fluor 750)

ISR-alpha Reactivité: Humain, Rat IF (cc), IF (p) Hôte: Lapin Polyclonal Alexa Fluor 750
N° du produit ABIN5005451
  • Antigène Voir toutes ISR-alpha Anticorps
    ISR-alpha (Insulin Receptor alpha (ISR-alpha))
    Épitope
    • 16
    • 14
    • 2
    • 2
    • 1
    AA 51-150
    Reactivité
    • 51
    • 41
    • 11
    • 2
    • 2
    • 2
    • 2
    Humain, Rat
    Hôte
    • 45
    • 6
    Lapin
    Clonalité
    • 33
    • 15
    • 3
    Polyclonal
    Conjugué
    • 14
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Cet anticorp ISR-alpha est conjugé à/à la Alexa Fluor 750
    Application
    • 26
    • 25
    • 19
    • 14
    • 11
    • 10
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
     Réactivité croisée
    Humain, Rat
    Homologie
    Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogène
    KLH conjugated synthetic peptide derived from human Insulin Receptor Alpha
    Isotype
    IgG
    Top Product
    Discover our top product ISR-alpha Anticorps primaire
  • Indications d'application
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Agent conservateur
    ProClin
    Précaution d'utilisation
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Stock
    -20 °C
    Stockage commentaire
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Date de péremption
    12 months
  • Antigène
    ISR-alpha (Insulin Receptor alpha (ISR-alpha))
    Autre désignation
    Insulin Receptor Alpha (ISR-alpha Produits)
    Synonymes
    anticorps CD220, anticorps HHF5, anticorps insulin receptor, anticorps INSR
    Sujet

    Synonyms: HHF5, CD220, Insulin receptor, IR, INSR

    Background: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: anti-apoptotic effect of insulin by inducing phosphorylation of BAD, regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K-AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway.

    ID gène
    3643
    UniProt
    P06213
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