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HSP70 anticorps (FITC)

HSP70 Reactivité: Humain WB, IF, ICC, FACS Hôte: Souris Monoclonal 1H11 FITC
N° du produit ABIN5066941
  • Antigène Voir toutes HSP70 Anticorps
    HSP70 (Heat Shock Protein 70 (HSP70))
    Reactivité
    • 323
    • 199
    • 165
    • 71
    • 62
    • 53
    • 52
    • 52
    • 36
    • 33
    • 29
    • 29
    • 28
    • 23
    • 20
    • 18
    • 15
    • 13
    • 12
    • 12
    • 10
    • 10
    • 9
    • 9
    • 9
    • 9
    • 7
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 206
    • 150
    • 13
    • 11
    • 11
    Souris
    Clonalité
    • 228
    • 162
    Monoclonal
    Conjugué
    • 112
    • 31
    • 28
    • 26
    • 24
    • 20
    • 19
    • 18
    • 18
    • 18
    • 12
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Cet anticorp HSP70 est conjugé à/à la FITC
    Application
    • 374
    • 159
    • 142
    • 122
    • 122
    • 110
    • 101
    • 66
    • 62
    • 61
    • 41
    • 33
    • 22
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    • 21
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Specificité
    Induced by heat shock.,Conformation-spec ab for mem-bound HSP70. Binds to cell surface HSP70 w/o permeabilization of cell mem in tumor cells. Does't bind to Intra-C HSP70 at low conc. X-reacts with HSC70. ~70 kDa in WB.
     Réactivité croisée
    Boeuf (Vache), Humain, Souris, Porc, Rat
    Purification
    Protein G Purified
    Immunogène
    Human native HSP70 protein
    Clone
    1H11
    Isotype
    IgG1
    Top Product
    Discover our top product HSP70 Anticorps primaire
  • Indications d'application
    • WB (1:1000)
    • ICC/IF (1:100)
    • FACS (1:250)
    • optimal dilutions for assays should be determined by the user.
    Commentaires

    A 1:250 dilution of ABIN5066941 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Conjugated antibodies should be stored at 4°C
  • Antigène
    HSP70 (Heat Shock Protein 70 (HSP70))
    Autre désignation
    HSP70 (HSP70 Produits)
    Synonymes
    anticorps APG-2, anticorps HS24/P52, anticorps HSPH2, anticorps RY, anticorps hsp70, anticorps hsp70RY, anticorps CG31354, anticorps HSP70, anticorps Hsp70Bb, anticorps hsp70B, anticorps hsp70Bb-prime, anticorps DmelCG5834, anticorps CG5834, anticorps HSPA1, anticorps HSP70B', anticorps HSPA6, anticorps ARABIDOPSIS HEAT SHOCK PROTEIN 70, anticorps ATHSP70, anticorps heat shock protein 70, anticorps LOC100305036, anticorps hsc70, anticorps Hsp70, anticorps Hsp70-1, anticorps Hsp70.1, anticorps hsp68, anticorps Hsp110, anticorps irp94, anticorps HSP70-2, anticorps HSPA1B, anticorps HSPA2, anticorps hsp70-5, anticorps HSP70-1, anticorps HSP70.1, anticorps HSP70.2, anticorps heat shock protein family A (Hsp70) member 4, anticorps CG5834 gene product from transcript CG5834-RA, anticorps heat shock protein 70, anticorps heat shock protein family A (Hsp70) member 6, anticorps heat shock 70kDa protein 2, anticorps heat shock 70 kD protein cognate, anticorps Hsp70 family chaperone, anticorps Heat shock protein 70, anticorps Heat shock protein 70, putative, anticorps heat shock protein 1B, anticorps heat shock protein family A member 4, anticorps heat shock 70kDa protein 1A, anticorps heat shock protein 1, anticorps Heat Shock Protein, anticorps heat shock cognate 70-kd protein, anticorps Heat shock 70 kDa protein 1A, anticorps HSPA4, anticorps Hsp70Bbb, anticorps HSP70, anticorps HSPA6, anticorps HSPA2, anticorps PCC7424_2419, anticorps Isop_1041, anticorps CGB_C3390W, anticorps Bacsa_1698, anticorps dnaK-B, anticorps LOC100305036, anticorps Hspa1b, anticorps Hspa4, anticorps HSPA1A, anticorps hsp1, anticorps hsp-70, anticorps hsp70, anticorps LOC108348108
    Sujet
    HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
    ID gène
    3303
    NCBI Accession
    NP_005336
    UniProt
    P0DMV8, P0DMV9
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