HSP70 anticorps (Heat Shock Protein 70) (PE-Atto 594)

Details for Product anti-HSP70 Antibody No. ABIN5066943
Antigène
  • APG-2
  • HS24/P52
  • HSPH2
  • RY
  • hsp70
  • hsp70RY
  • CG31354
  • HSP70
  • Hsp70Bb
  • hsp70B
  • hsp70Bb-prime
  • DmelCG5834
  • CG5834
  • HSPA1
  • HSP70B'
  • HSPA6
  • ARABIDOPSIS HEAT SHOCK PROTEIN 70
  • ATHSP70
  • heat shock protein 70
  • LOC100305036
  • hsc70
  • Hsp70
  • Hsp70-1
  • Hsp70.1
  • hsp68
  • Hsp110
  • irp94
  • HSP70-2
  • HSPA1B
  • HSPA2
  • hsp70-5
  • heat shock protein family A (Hsp70) member 4
  • CG5834 gene product from transcript CG5834-RA
  • heat shock protein 70
  • heat shock protein family A (Hsp70) member 6
  • heat shock 70kDa protein 2
  • heat shock 70 kD protein cognate
  • Hsp70 family chaperone
  • Heat shock protein 70
  • Heat shock protein 70, putative
  • heat shock protein 1B
  • heat shock protein family A member 4
  • heat shock 70kDa protein 1A
  • heat shock protein 1
  • Heat Shock Protein
  • heat shock cognate 70-kd protein
  • HSPA4
  • Hsp70Bbb
  • HSP70
  • HSPA6
  • HSPA2
  • PCC7424_2419
  • Isop_1041
  • CGB_C3390W
  • Bacsa_1698
  • dnaK-B
  • LOC100305036
  • Hspa1b
  • Hspa4
  • HSPA1A
  • hsp1
  • hsp-70
  • hsp70
Reactivité
Boeuf (Vache), Humain, Souris, Porc, Rat (Rattus)
911
706
705
316
283
276
260
243
189
182
145
143
137
132
131
94
84
74
64
63
59
49
44
44
43
42
42
26
18
18
17
8
7
6
5
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Hôte
Souris
513
451
64
42
42
Clonalité (Clone)
Monoclonal ()
Conjugué
Cet anticorp HSP70 est conjugé à/à la PE-Atto 594
53
51
40
38
38
36
35
35
35
35
35
35
35
35
35
35
25
15
13
13
13
13
13
9
8
7
7
7
6
6
5
5
5
5
4
3
3
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
1012
462
462
368
361
335
208
156
145
96
74
62
42
23
11
9
3
2
2
1
1
Options
Immunogène Human native HSP70 protein
Clone 1H11
Isotype IgG1
Specificité Conformation-specific antibody for membrane-bound HSP70. Binds to cell surface HSP70 without permeabilization of cell membrane in tumor cell lines. Does not bind to intracellular HSP70 at low concentrations. Cross-reacts with HSC70. Detects a ~70 kDa band in Western Blot.
Purification Protein G Purified
Antigène
Autre désignation HSP70 (HSP70 Antibody Extrait)
Sujet HSP70 is a highly conserved protein that is ubiquitously expressed. It can be found in chloroplasts, eukaryotic cytosol, endoplasmic reticulum, and mitochondria, but also embedded in the cell membrane and in the extracellular space (1,2,3,4). Even though HSP70 is one of the most studied heat shock proteins, the export mechanism and method of membrane insertion are not fully understood. The majority of the proteins in the heat shock family lack a consensus signal for secretion via the ER-Golgi pathway (5). Researchers have found that HSP70 may be released from cells via exosomes or secretory vesicles (6). Although HSP70 is ubiquitously expressed, there is not much information about its presence on cell surface. The finding that HSP70 is localized on the cell surface of cancer cells but not normal cells suggests a conformational change of HSP70 in the lower pH environment characteristic of cancer cells (7). The presence of cell membrane embedded HSP70 has been found to increase the stability of cancer cells, thereby protecting tumors from environmental stress (8, 9). Anti-HSP70 antibody, clone 1H11 (Catalog No. SMC-249) is unique from other commercially available antibodies in that it can bind to the extracellular region of the cell membrane embedded HSP70 protein, allowing researchers to differentiate between membrane bound and intracellular HSP70 across cancer cells types.
ID gène 3303
NCBI Accession NP_005336
Indications d'application
  • WB (1:1000)
  • ICC/IF (1:100)
  • FACS (1:250)
  • optimal dilutions for assays should be determined by the user.
Commentaires

A 1:250 dilution of SMC-249 was sufficient for detection of cell surface HSP70 in HCT116 cells using Fluorescence-activated cell sorting, applied in flow cytometry, with FITC as the fluorescent probe.

Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % Sodium Azide
Agent conservateur Sodium azide
Précaution d'utilisation This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock -20 °C
Images (Fournisseur)
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Immunocytochemistry/Immunofluorescence analysis on non-permeabilized HCT116 cells usi...
Flow Cytometry (FACS) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Fluorescence-activated cell sorting (FACS) using Mouse Anti-HSP70 Monoclonal Antibody...
Western Blotting (WB) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Western Blot analysis of Human Heat shocked HeLa cell lysates showing detection of HS...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Immunocytochemistry/Immunofluorescence analysis on permeabilized HCT116 cells using M...
Flow Cytometry (FACS) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Fluorescence-activated cell sorting analysis using Mouse Anti-HSP70 Monoclonal Antibo...
Flow Cytometry (FACS) image for anti-Heat Shock Protein 70 (HSP70) antibody (PE-Atto 594) (ABIN5066943) Fluorescence-activated cell sorting analysis using Mouse Anti-HSP70 Monoclonal Antibo...
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