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CD4 anticorps (FITC)

CD4 Reactivité: Souris FACS Hôte: Rat Monoclonal GK1-5 FITC
N° du produit ABIN509566
  • Antigène Voir toutes CD4 Anticorps
    CD4
    Reactivité
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    • 120
    • 38
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    Souris
    Hôte
    • 361
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    • 4
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    Rat
    Clonalité
    • 501
    • 138
    • 3
    Monoclonal
    Conjugué
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    Cet anticorp CD4 est conjugé à/à la FITC
    Application
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    Flow Cytometry (FACS)
    Specificité
    The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).
    Réactivité croisée (Details)
    Mouse
    Purification
    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    Immunogène
    Mouse CTL clone V4 cells
    Clone
    GK1-5
    Isotype
    IgG2b
    Top Product
    Discover our top product CD4 Anticorps primaire
  • Indications d'application
    Flow cytometry: Recommended dilution: 1-2 μg/mL.
    Commentaires

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze.
    Avoid prolonged exposure to light.
    Stock
    4 °C
    Stockage commentaire
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Hu, Watson, Zhang, Graf, Wang, Sartor, Howden, Fletcher, Alexander: "Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 10, pp. 6593-603, (2008) (PubMed).

    Yi, Zhen, Zeng, Zhang, Zhao: "Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice." dans: Transplantation, Vol. 85, Issue 8, pp. 1167-74, (2008) (PubMed).

    Felix, Donermeyer, Horvath, Walters, Gross, Suri, Allen: "Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes." dans: Nature immunology, Vol. 8, Issue 4, pp. 388-97, (2007) (PubMed).

    Zheng, Han, Kelsoe: "T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1." dans: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 1083-91, (1997) (PubMed).

    Gavett, Chen, Finkelman, Wills-Karp: "Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia." dans: American journal of respiratory cell and molecular biology, Vol. 10, Issue 6, pp. 587-93, (1994) (PubMed).

    Godfrey, Kennedy, Gately, Hakimi, Hubbard, Zlotnik: "IL-12 influences intrathymic T cell development." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2729-35, (1994) (PubMed).

    Wu, Antica, Johnson, Scollay, Shortman: "Developmental potential of the earliest precursor cells from the adult mouse thymus." dans: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, (1992) (PubMed).

    Dialynas, Quan, Wall, Pierres, Quintáns, Loken, Pierres, Fitch: "Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 131, Issue 5, pp. 2445-51, (1983) (PubMed).

    Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." dans: Immunological reviews, Vol. 74, pp. 29-56, (1983) (PubMed).

  • Antigène
    CD4
    Autre désignation
    CD4 (CD4 Produits)
    Synonymes
    anticorps cd4, anticorps L3T4, anticorps Ly-4, anticorps CD4mut, anticorps W3/25, anticorps p55, anticorps CD4 molecule, anticorps carcinoembryonic antigen-related cell adhesion molecule 5, anticorps T-cell surface glycoprotein CD4, anticorps CD4 antigen, anticorps Cd4 molecule, anticorps CD4, anticorps LOC100125525, anticorps cd4, anticorps Cd4
    Sujet
    CD4 Molecule,CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).,T4/Leu-3, L3T4
    ID gène
    12504
    UniProt
    P06332
    Pathways
    TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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