Mouse monoclonal antibody raised against recombinant HLA-E.
Specificité
This antibody recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results showed that This antibody cross-reacts with some classical MHC ClassI molecules (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that This antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32,-B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.
The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
In TBS, pH 8.0 (0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store at 4°C. Do not freeze.
Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).
Palmisano, Contardi, Morabito, Gargaglione, Ferrara, Pistillo: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." dans: Human immunology, Vol. 66, Issue 1, pp. 1-12, (2004) (PubMed).
Menier, Saez, Horejsi, Martinozzi, Krawice-Radanne, Bruel, Le Danff, Reboul, Hilgert, Rabreau, Larrad, Pla, Carosella, Rouas-Freiss: "Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules." dans: Human immunology, Vol. 64, Issue 3, pp. 315-26, (2003) (PubMed).
Antigène
HLA-E
(HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))