Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FACS)
Fonction
Mouse monoclonal antibody raised against partial recombinant LAT2.
Specificité
This antibody reacts with Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25-30 KDa transmembrane adaptor protein present in membrane microdomains (rafts) of B lymphocytes, NK cells and myeloid cells.
Réactivité croisée
Humain, Souris
Immunogène
Recombinant protein corresponding to partial human LAT2.
LAT2
Reactivité: Humain
IHC (p), IF (p)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Flow Cytometry (1:30) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.1 mg/mL
Buffer
In PBS (0.2 % BSA, 0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store in the dark at 4°C. Do not freeze. Avoid prolonged exposure to light. Aliquot to avoid repeated freezing and thawing.
Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." dans: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, (2007) (PubMed).
Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." dans: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).
Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." dans: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).
Antigène
LAT2
(Linker For Activation of T Cells Family, Member 2 (LAT2))