ELISA (1:4000-1:20000) Western Blot (1:500-1:3000) Immunohistochemistry (1:500-1:3000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Lyophilized
Buffer
Lyophilized from 0.02 M KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide).
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
McDonald, El-Deiry: "Suppression of caspase-8- and -10-associated RING proteins results in sensitization to death ligands and inhibition of tumor cell growth." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 16, pp. 6170-5, (2004) (PubMed).
Coumailleau, Das, Alcover, Raposo, Vandormael-Pournin, Le Bras, Baldacci, Dautry-Varsat, Babinet, Cohen-Tannoudji: "Over-expression of Rififylin, a new RING finger and FYVE-like domain-containing protein, inhibits recycling from the endocytic recycling compartment." dans: Molecular biology of the cell, Vol. 15, Issue 10, pp. 4444-56, (2004) (PubMed).
Tibbetts, Shiozaki, Gu, McDonald, El-Deiry, Shi: "Crystal structure of a FYVE-type zinc finger domain from the caspase regulator CARP2." dans: Structure (London, England : 1993), Vol. 12, Issue 12, pp. 2257-63, (2004) (PubMed).
Antigène
RFFL
(Ring Finger and FYVE-Like Domain Containing 1 (RFFL))