Western Blot (1:1000-1:2000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Wu, Chai, Suber, Wu, Du, Wang, Shi: "Structural basis of IAP recognition by Smac/DIABLO." dans: Nature, Vol. 408, Issue 6815, pp. 1008-12, (2001) (PubMed).
Du, Fang, Li, Li, Wang: "Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition." dans: Cell, Vol. 102, Issue 1, pp. 33-42, (2000) (PubMed).
Srinivasula, Datta, Fan, Fernandes-Alnemri, Huang, Alnemri: "Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway." dans: The Journal of biological chemistry, Vol. 275, Issue 46, pp. 36152-7, (2000) (PubMed).
Antigène
DIABLO
(Second Mitochondria-Derived Activator of Caspase (DIABLO))