Immunofluorescence (1:25) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:100) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Chen, Riley, Zheng, Chen, Lee: "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation." dans: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49408-16, (2002) (PubMed).
Eto, Elliott, Prickett, Brautigan: "Inhibitor-2 regulates protein phosphatase-1 complexed with NimA-related kinase to induce centrosome separation." dans: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44013-20, (2002) (PubMed).
Schutte, Bjork, Coppage, Malik, Gregory, Scott, Brentzell, Watanabe, Dixon, Murray: "A preliminary gene map for the Van der Woude syndrome critical region derived from 900 kb of genomic sequence at 1q32-q41." dans: Genome research, Vol. 10, Issue 1, pp. 81-94, (2000) (PubMed).
Antigène
NEK2
(NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))