ELISA (1:1000) Western Blot (1:100-500) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In PBS (0.09 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Wang, Grammatikakis, Siganou, Calderwood: "Regulation of molecular chaperone gene transcription involves the serine phosphorylation, 14-3-3 epsilon binding, and cytoplasmic sequestration of heat shock factor 1." dans: Molecular and cellular biology, Vol. 23, Issue 17, pp. 6013-26, (2003) (PubMed).
Hilgarth, Hong, Park-Sarge, Sarge: "Insights into the regulation of heat shock transcription factor 1 SUMO-1 modification." dans: Biochemical and biophysical research communications, Vol. 303, Issue 1, pp. 196-200, (2003) (PubMed).
He, Soncin, Grammatikakis, Li, Siganou, Gong, Brown, Kingston, Calderwood: "Elevated expression of heat shock factor (HSF) 2A stimulates HSF1-induced transcription during stress." dans: The Journal of biological chemistry, Vol. 278, Issue 37, pp. 35465-75, (2003) (PubMed).