ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Fonction
Goat polyclonal antibody raised against synthetic peptide of ADAM12.
Séquence
AARPLPVSPA RALC
Specificité
This antibody is expected to recognize both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS.
Réactivité croisée
Humain
Attributs du produit
Antibody Reactive Against Synthetic Peptide.
Immunogène
A synthetic peptide corresponding to human ADAM12.
ADAM12
Reactivité: Humain, Souris
ELISA, IHC
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA (1:64000) Western blot (0.1-0.3 μg/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 μg/mL) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.5 mg/mL
Buffer
In Tris saline, pH 7.3 (0.5 % BSA, 0.02 % sodium azide)
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C
Stockage commentaire
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Galliano, Huet, Frygelius, Polgren, Wewer, Engvall: "Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion." dans: The Journal of biological chemistry, Vol. 275, Issue 18, pp. 13933-9, (2000) (PubMed).