Immunohistochemistry (1:1000) Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50 % glycerol, 100 μg/mL BSA).
Stock
-20 °C,-80 °C
Stockage commentaire
Store at -20°C for short term storage is acceptable as aliquots may be taken without freeze/thaw due to the presence of 50% glycerol. For long term storage, store at -80°C. Aliquot to avoid repeated freezing and thawing.
Kumar, Tang, Ravindranath, Clark, Croze, Fuchs: "SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor." dans: The EMBO journal, Vol. 22, Issue 20, pp. 5480-90, (2003) (PubMed).
Kirkwood: "Cancer immunotherapy: the interferon-alpha experience." dans: Seminars in oncology, Vol. 29, Issue 3 Suppl 7, pp. 18-26, (2002) (PubMed).
Biron: "Interferons alpha and beta as immune regulators--a new look." dans: Immunity, Vol. 14, Issue 6, pp. 661-4, (2001) (PubMed).