Rabbit polyclonal antibody raised against synthetic phosphopeptide of Pak2.
Specificité
Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with lambda phosphatase.
Réactivité croisée
Boeuf (Vache), Chien, Humain, Souris, Rat
Attributs du produit
Antibody Reactive Against Synthetic Peptide.
Immunogène
Synthetic phosphopeptide corresponding to residues surrounding phospho-T402 of rat Pak2.
PAK2
Reactivité: Humain
WB, ELISA
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Restrictions
For Research Use only
Format
Liquid
Buffer
In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50 % glycerol, 100 μg BSA per mL).
Stock
-20 °C
Stockage commentaire
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Boda, Alberi, Nikonenko, Node-Langlois, Jourdain, Moosmayer, Parisi-Jourdain, Muller: "The mental retardation protein PAK3 contributes to synapse formation and plasticity in hippocampus." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 48, pp. 10816-25, (2004) (PubMed).
Jakobi, McCarthy, Koeppel, Stringer: "Caspase-activated PAK-2 is regulated by subcellular targeting and proteasomal degradation." dans: The Journal of biological chemistry, Vol. 278, Issue 40, pp. 38675-85, (2003) (PubMed).
Jakobi, Huang, Walter, Tuazon, Traugh: "Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation." dans: European journal of biochemistry / FEBS, Vol. 267, Issue 14, pp. 4414-21, (2000) (PubMed).