Tyrosine Hydroxylase anticorps

N° du produit ABIN372734

Détail du produit anti-Tyrosine Hydroxylase anticorps

Antigène: Tyrosine Hydroxylase (TH)

Reactivité: Mammifères

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Tyrosine Hydroxylase est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Specificité: Specific for the ~60k tyrosine hydroxylase protein. The antibody recognizes all mammalian and at least some non-mammalian forms of the enzyme in Western blots and in Immunohistochemistry/Immunofluorescence.

Réactivité croisée (Details): Species reactivity (tested):Mammalia.

Purification: Affinity Chromatography on Protein A.

Immunogène: SDS-denatured rat tyrosine hydroxylase, purified from pheochromocytoma.

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 1/1,000. Immunofluorescence 1/1000 (Frozen Sections). Immunohistochemistry on Frozen Sections: 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C/-80 °C

Stockage commentaire: Store the antibody undiluted (in aliquots) at-20 °C or (in aliquots) at -80 °C for long term storage.

Références pour anticorps anti-Tyrosine Hydroxylase (ABIN372734)

Grapensparr, Vasylovska, Li, Olerud, Jansson, Kozlova, Carlsson: "Co-transplantation of human pancreatic islets with post-migratory neural crest stem cells increases β-cell proliferation and vascular and neural regrowth." dans: The Journal of clinical endocrinology and metabolism, Vol. 100, Issue 4, pp. E583-90, (2015) (PubMed).

Kirane, Toombs, Larsen, Ostapoff, Meshaw, Zaknoen, Brekken, Burrows: "Epithelial-mesenchymal transition increases tumor sensitivity to COX-2 inhibition by apricoxib." dans: Carcinogenesis, Vol. 33, Issue 9, pp. 1639-46, (2012) (PubMed).

Kish, Kalasinsky, Derkach, Schmunk, Guttman, Ang, Adams, Furukawa, Haycock: "Striatal dopaminergic and serotonergic markers in human heroin users." dans: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 24, Issue 5, pp. 561-7, (2001) (PubMed).

Salvatore, Waymire, Haycock: "Depolarization-stimulated catecholamine biosynthesis: involvement of protein kinases and tyrosine hydroxylase phosphorylation sites in situ." dans: Journal of neurochemistry, Vol. 79, Issue 2, pp. 349-60, (2001) (PubMed).

Witkovsky, Gabriel, Haycock, Meller: "Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina." dans: Journal of chemical neuroanatomy, Vol. 19, Issue 2, pp. 105-16, (2000) (PubMed).

Zhu, Klimek, Haycock, Ordway: "Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain." dans: Journal of neuroscience methods, Vol. 99, Issue 1-2, pp. 37-44, (2000) (PubMed).

Détails sur Tyrosine Hydroxylase

Antigène: Tyrosine Hydroxylase (TH)

Autre désignation: Tyrosine 3-Monooxygenase (TH) (TH Produits)

Synonymes: anticorps DYT14, anticorps DYT5b, anticorps TYH, anticorps The, anticorps CG10118, anticorps DH65B, anticorps DTH, anticorps Dmel\\CG10118, anticorps Pale, anticorps Ple, anticorps TH, anticorps Th, anticorps VII, anticorps dTH1, anticorps dTH65B, anticorps pale/ple, anticorps th, anticorps dyt14, anticorps dyt5b, anticorps tyh, anticorps tyrosine hydroxylase, anticorps pale, anticorps tyrosine 3-monooxygenase, anticorps tyrosine hydroxylase S homeolog, anticorps TH, anticorps Th, anticorps ple, anticorps th, anticorps th.S, anticorps LOC100008895

Sujet: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001, Dunkley et al., 2004).Synonyms: TYH, Tyrosine 3-hydroxylase

ID gène: 25085

NCBI Accession: NP_036872

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour