RFFL anticorps (N-Term)

N° du produit ABIN401034

Détail du produit anti-RFFL anticorps

Antigène: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Épitope: AA 1-363, N-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RFFL est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against RFFL protein.

Réactivité croisée (Details): Species reactivity (expected):Canine, Rat, Mouse, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography on Protein A

Immunogène: Recombinant protein corresponding to amino acids 1-363 of human RFFL protein

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/4000 - 1/20000. Western Blot: 1/500 - 1/3000. Immunohistochemistry: 1/500 - 1/3000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Détails sur RFFL

Antigène: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Autre désignation: RFFL / RNF189 (RFFL Produits)

Synonymes: anticorps MGC84042, anticorps RFFL, anticorps Rffl, anticorps 1700051E09Rik, anticorps 4930516L10Rik, anticorps BG080975, anticorps Carp2, anticorps CARP-2, anticorps CARP2, anticorps FRING, anticorps RIFIFYLIN, anticorps RNF189, anticorps RNF34L, anticorps ring finger and FYVE like domain containing E3 ubiquitin protein ligase, anticorps ring finger and FYVE-like domain containing E3 ubiquitin protein ligase L homeolog, anticorps ring finger and FYVE-like domain containing 1, anticorps ring finger and FYVE like domain containing protein, anticorps ring finger and FYVE-like domain containing E3 ubiquitin protein ligase, anticorps RFFL, anticorps rffl.L, anticorps Rffl

Sujet: RFFL, also known as Rififylin, RING finger and FYVE-like domaincontaining protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kappaB activation. RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.Synonyms: CARP-2, CARP2, Caspases-8 and -10-associated RING finger protein 2, E3 ubiquitin-protein ligase rififylin, FYVE-RING finger protein Sakura, Fring, RIFIFYLIN, RING finger protein 189, RING finger protein 34-like, RNF34L

ID gène: 117584

NCBI Accession: NP_001017368

UniProt: Q8WZ73