CDC27 anticorps (pThr244)

N° du produit ABIN401313

Détail du produit anti-CDC27 anticorps

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Épitope: AA 239-249, pThr244

Reactivité: Humain, Rat, Chien, Boeuf (Vache), Chimpanzé

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC27 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody is directed against the phosphorylated form of CDC27 at the pT244 residue.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine, Chicken, Chimpanzee, Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogène: Synthetic peptide corresponding aa 239-249 of Human CDC27

Isotype: IgG

Information d'application

Indications d'application: ELISA: 1/8,000 - 1/30,000. Western Blot: 1/300 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Détails sur CDC27

Antigène: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Autre désignation: CDC27 (CDC27 Produits)

Synonymes: anticorps ANAPC3, anticorps APC3, anticorps CDC27Hs, anticorps D0S1430E, anticorps D17S978E, anticorps HNUC, anticorps NUC2, anticorps wu:fb54g06, anticorps wu:fc88c12, anticorps AI452358, anticorps BC023187, anticorps CDC27, anticorps NV10210, anticorps cell division cycle 27, anticorps cell division cycle 27 S homeolog, anticorps cell division cycle protein 27 homolog, anticorps anaphase-promoting complex subunit Apc3, anticorps CDC27, anticorps cdc27, anticorps Cdc27, anticorps cdc27.S, anticorps LOC100123129, anticorps nuc2

Sujet: Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis.Synonyms: CDC27Hs, Cell division cycle protein 27 homolog, D0S1430E, D17S978E, H-NUC

ID gène: 996

NCBI Accession: NP_001107563

UniProt: P30260