SIRT4 anticorps (C-Term)

N° du produit ABIN374409

Détail du produit anti-SIRT4 anticorps

Antigène: SIRT4 (Sirtuin 4 (SIRT4))

Épitope: C-Term

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SIRT4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: SRCGELLPLI DPC

Specificité: This antibody detects SIRT4 at C-term.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from C-Terminus of the protein sequence according to NP_036372.1 Genename: SIRT4

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in Human Kidney and U937 lysates(calculated MW of 35.8 kDa according to NP_036372.1). In transfected HEK293 transientlyexpressing SIRT4 a band of approx.38 kDa is observed. This band is not observed in thenon-transfected HEK293. Recommended Concentration: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: Skeletal Muscle shows spotty staining ofstriated fibres in longitudinal section. Recommended Concentration: 2-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-SIRT4 (ABIN374409)

Ott, Plitzko, Krischkowski, Reichmann, Bittner, Mendel, Kunze, Clement, Havemeyer: "Reduction of sulfamethoxazole hydroxylamine (SMX-HA) by the mitochondrial amidoxime reducing component (mARC)." dans: Chemical research in toxicology, Vol. 27, Issue 10, pp. 1687-95, (2015) (PubMed).

Ahuja, Schwer, Carobbio, Waltregny, North, Castronovo, Maechler, Verdin: "Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase." dans: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33583-92, (2007) (PubMed).

Détails sur SIRT4

Antigène: SIRT4 (Sirtuin 4 (SIRT4))

Autre désignation: SIRT4 (SIRT4 Produits)

Synonymes: anticorps SIRT4, anticorps im:7139453, anticorps SIR2L4, anticorps 4930596O17Rik, anticorps sirtuin 4, anticorps SIRT4, anticorps sirt4, anticorps Sirt4

Sujet: SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.Synonyms: NAD-Dependent ADP-Ribosyltransferase Sirtuin-4, SIR2-like 4, SIR2L4, SIRT-4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin 4, sirtuin type 4, sirtuin-4

ID gène: 23409

NCBI Accession: NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion