PPIA anticorps (Internal Region)

N° du produit ABIN374639

Détail du produit anti-PPIA anticorps

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp PPIA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Séquence: C-ERFGSRNGKT SKK

Specificité: This antibody is expected to recognise Cyclophilin A. This product is expected to recognise PPIAL (NP_001008741.1, GeneID: 388817) which is identical to PPIA (NP_066953.1).

Réactivité croisée (Details): Species reactivity (expected):Mouse, Rat, Canine, Porcine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_066953.1. Genename: PPIA

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-0.3 μg/mL. Approx 18 kDa band observed in Human Lymph Node lysates(calculated MW 18.8 kDa according to NP_066953.1).

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur PPIA

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Autre désignation: Cyclophilin A (PPIA Produits)

Synonymes: anticorps Cyp1, anticorps PPIA-2, anticorps fa93g09, anticorps ppia, anticorps wu:fa93g09, anticorps wu:fb05e11, anticorps wu:fb13h02, anticorps wu:fb15a05, anticorps CYPA, anticorps CypA, anticorps Cypa, anticorps CYPH, anticorps CYCA, anticorps CyP-A, anticorps MGC75715, anticorps 2700098C05, anticorps Cphn, anticorps CyP-18, anticorps MT-ND1, anticorps MTND1, anticorps NADH1, anticorps ND1, anticorps cypA, anticorps PPIase A, anticorps ppial, anticorps wu:fj18g05, anticorps zgc:73102, anticorps zgc:86688, anticorps peptidylprolyl isomerase A, anticorps peptidylprolyl isomerase Aa (cyclophilin A), anticorps peptidylprolyl isomerase A (cyclophilin A) L homeolog, anticorps cyclophilin A, anticorps cyclophilin a, anticorps peptidylprolyl isomerase A (cyclophilin A), anticorps peptidylprolyl isomerase Ab (cyclophilin A), anticorps PPIA, anticorps ppiaa, anticorps ppia.L, anticorps Cypa, anticorps CNB01230, anticorps CNB01290, anticorps Tc00.1047053506925.300, anticorps Tb11.03.0250, anticorps CYPA, anticorps Ppia, anticorps ppia, anticorps ppiab

Sujet: Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.Synonyms: CYPA, Cyclophilin-A, Cyclosporin A-binding protein, EC=5.2.1.8, PPIA, PPIase A, Peptidyl-prolyl cis-trans isomerase A, Rotamase A

ID gène: 5478

NCBI Accession: NP_066953

UniProt: P62937