AIRE anticorps (Internal Region, Isoform 1)

N° du produit ABIN374692

Détail du produit anti-AIRE anticorps

Antigène: AIRE (Autoimmune Regulator (AIRE))

Épitope: Internal Region, Isoform 1

Reactivité: Humain, Rat, Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp AIRE est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Séquence: C-KDVDLSQPRK GRKP

Specificité: This antibody is expected to recognise isoform 1 (NP_000374.1) only.

Réactivité croisée (Details): Species reactivity (expected):Rat, Canine and Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_000374.1.

Information d'application

Indications d'application: Peptide ELISA: 1/32000. Western blot: 0.5-1.0 μg/mL. Immunohistochemistry: 2.5 μg/mL. In paraffin embedded Human Thymus shows nuclear staining in select thymocytes.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Références pour anticorps anti-AIRE (ABIN374692)

Nakasatomi, Maeshima, Mishima, Ikeuchi, Sakairi, Kaneko, Hiromura, Nojima: "Novel approach for the detection of tubular cell migration into the interstitium during renal fibrosis in rats." dans: Fibrogenesis & tissue repair, Vol. 8, pp. 12, (2015) (PubMed).

Ströbel, Hartmann, Rosenwald, Kalla, Ott, Friedel, Schalke, Kasahara, Tomaru, Marx: "Corticomedullary differentiation and maturational arrest in thymomas." dans: Histopathology, Vol. 64, Issue 4, pp. 557-66, (2014) (PubMed).

Jüngling, Liu, Lesting, Coulon, Sosulina, Reinscheid, Pape: "Activation of neuropeptide S-expressing neurons in the locus coeruleus by corticotropin-releasing factor." dans: The Journal of physiology, Vol. 590, Issue Pt 16, pp. 3701-17, (2012) (PubMed).

Wei, Akerman, Newey, Pan, Clinch, Jacob, Shen, Wilkins, Ellory: "The potassium-chloride cotransporter 2 promotes cervical cancer cell migration and invasion by an ion transport-independent mechanism." dans: The Journal of physiology, Vol. 589, Issue Pt 22, pp. 5349-59, (2011) (PubMed).

Yuan, Zhu, Zhou, Chen, Zhu, Ma, He, Tian, Shi: "Role of mast cell activation in inducing microglial cells to release neurotrophin." dans: Journal of neuroscience research, Vol. 88, Issue 6, pp. 1348-54, (2010) (PubMed).

Zelano, Berg, Thams, Hailer, Cullheim: "SynCAM1 expression correlates with restoration of central synapses on spinal motoneurons after two different models of peripheral nerve injury." dans: The Journal of comparative neurology, Vol. 517, Issue 5, pp. 670-82, (2009) (PubMed).

Détails sur AIRE

Antigène: AIRE (Autoimmune Regulator (AIRE))

Autre désignation: AIRE / APECED (AIRE Produits)

Synonymes: anticorps AIRE1, anticorps APECED, anticorps APS1, anticorps APSI, anticorps PGA1, anticorps autoimmune regulator, anticorps autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy), anticorps AaeL_AAEL013794, anticorps CpipJ_CPIJ001208, anticorps CpipJ_CPIJ001209, anticorps AIRE, anticorps Aire

Sujet: AIRE is a transcriptional regulator that is thought to be important in controlling tissue-specific expression of genes in the thymus. The precise function of the protein is not well defined. It contains zinc finger motifs and isoform 1 is localized to both the nucleus and cytoplasm. Three splice variant mRNAs products have been described. The longer AIRE1 mRNA appears to be more abundant and includes exons 1 through 14. Splice variant AIRE2 includes a portion of the non-coding region of exon 1, an alternatively spliced longer exon 8, plus exons 9 through 14. Variant AIRE3 includes the same exon 1-8-9 sequences as found in AIRE2 but utilizes additional alternative splicing in exon 10 that shifts the reading frame such that a stop codon in exon 12 is utilized. The resulting protein products of these splice variants differ significantly. Defects in this gene cause the autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).Synonyms: Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, Autoimmune regulator

ID gène: 326, 9606

UniProt: O43918

Pathways: Chromatin Binding