EBF1 anticorps (Internal Region)

N° du produit ABIN499164

Détail du produit anti-EBF1 anticorps

Antigène: EBF1 (Early B-Cell Factor 1 (EBF1))

Épitope: Internal Region

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EBF1 est non-conjugé

Application: Western Blotting (WB)

Séquence: C-HNQLPALANT SV

Specificité: This antibody detects EBF1 / COE1 (Internal region). It is expected not to cross-react with EBF2, EBF3 and EBF4.

Réactivité croisée (Details): Species reactivity (expected):Mouse, ratSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the internal region of the protein sequence according to NP_076870.1

Information d'application

Indications d'application: Peptide ELISA: limit dilution 1: 64000. Western blot: 0.3-0.1 μg/mL, approx.75 kDa band observed in Human Adipose lysates. Anonymous customer used this product successfully in IP from induced 3T3-L1 expressingFLAG-EBF1.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Détails sur EBF1

Antigène: EBF1 (Early B-Cell Factor 1 (EBF1))

Autre désignation: EBF1 / COE1 (EBF1 Produits)

Synonymes: anticorps EBF1, anticorps 9830166E18Rik, anticorps AW990631, anticorps Ebf, anticorps O/E-1, anticorps Olf-1, anticorps Olf1, anticorps COE1, anticorps EBF, anticorps OLF1, anticorps early B-cell factor 1, anticorps early B cell factor 1, anticorps EBF1, anticorps Ebf1

Sujet: EBF1 belongs to the COE family. It contains 1 IPT/TIG domain. EBF1 is a transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. Forms either a homodimer or a heterodimer with a related family member. Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.Synonyms: EBF, Early B-cell factor, OE-1, Transcription factor COE1

ID gène: 1879

NCBI Accession: NP_076870

UniProt: Q9UH73