Recombinant HIF1A anticorps
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- Antigène Voir toutes HIF1A Anticorps
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Type d'anticorp
- Recombinant Antibody
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Reactivité
- Humain, Souris, Rat, Boeuf (Vache)
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp HIF1A est non-conjugé
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Application
- ELISA
- Marque
- AbFlex®
- Specificité
- Bovine, Human, Mouse, Rat
- Attributs du produit
- AbFlex™ HIF-1 alpha antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. HIF-1 (hypoxia inducible factor 1, alpha subunit, MOP1, PASD8, bHLHe78, PAS domain-containing protein 8) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas. Two alternative transcripts encoding different isoforms have been identified.
- Purification
- Ni-NTA
- Isotype
- IgG2a
- Top Product
- Discover our top product HIF1A Anticorps primaire
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- Indications d'application
- Bead-based ELISA: 10 - 76 ng/mL
- Commentaires
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AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.
AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A. - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
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- Antigène
- HIF1A (Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A))
- Autre désignation
- HIF-1 alpha (HIF1A Produits)
- Synonymes
- anticorps hif1a, anticorps AA959795, anticorps HIF1alpha, anticorps MOP1, anticorps bHLHe78, anticorps HIF-1A, anticorps HIF-1alpha, anticorps HIF1, anticorps HIF1-ALPHA, anticorps PASD8, anticorps HIF1-alpha, anticorps hif-1a, anticorps hypoxia inducible factor 1 alpha, anticorps hypoxia inducible factor 1, alpha subunit, anticorps hypoxia inducible factor 1 alpha subunit, anticorps HIF (Hypoxia Inducible Factor) homologa, anticorps hif-1a, anticorps Hif1a, anticorps HIF1A, anticorps hif1a, anticorps hif-1
- Poids moléculaire
- 100 kDa
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals, L'effet Warburg
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