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Fibronectin anticorps (Biotin)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Singe WB, IHC, ELISA, IP Hôte: Lapin Polyclonal Biotin
N° du produit ABIN5596762
  • Antigène Voir toutes Fibronectin Anticorps
    Fibronectin
    Reactivité
    • 107
    • 57
    • 57
    • 24
    • 14
    • 10
    • 5
    • 2
    • 1
    • 1
    • 1
    Humain, Souris, Rat, Boeuf (Vache), Singe
    Hôte
    • 72
    • 61
    • 5
    • 1
    Lapin
    Clonalité
    • 72
    • 66
    Polyclonal
    Conjugué
    • 62
    • 18
    • 9
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp Fibronectin est conjugé à/à la Biotin
    Application
    • 72
    • 41
    • 39
    • 33
    • 26
    • 20
    • 18
    • 18
    • 18
    • 17
    • 14
    • 13
    • 10
    • 7
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with human Fibronectin and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross-reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
    Purification
    Anti-fibronectin (rabbit) antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificity.
    Immunogène

    Immunogen: Fibronectin was purified from Human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration. Immunization occurred after single-band purity was assessed by SDS-PAGE.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Labeling Ratio
    10-20
    Top Product
    Discover our top product Fibronectin Anticorps primaire
    Informations sur le produit

    Cet anticorps polyclonal de lapin anti-fibronectine est conjugué à la biotine et convient à la détection de la fibronectine (FN, FN1) chez l'homme, la souris, le rat, le bovin et le singe. L'anticorps anti-fibronectine est validé pour l'ELISA, l'immunohistochimie, l'immunoprécipitation et le western blotting. Quatre images du produit pour l'IHC et le profil de dépliage sont actuellement disponibles comme preuve de la performance de l'anticorps. Utilisez notre anticorps de haute qualité contre la Fibronectine pour détecter de manière fiable la Fibronectine pour une variété d'espèces.

    Les fibronectines se lient aux surfaces cellulaires et à divers composés dont le collagène, la fibrine, l'héparine, l'ADN et l'actine. Les fibronectines sont impliquées dans l'adhésion cellulaire, la motilité cellulaire, l'opsonisation, la cicatrisation et le maintien de la forme des cellules. Impliquée dans la compaction des ostéoblastes par le processus d'assemblage matriciel médié par les cellules de fibronectine, essentiel pour la minéralisation des ostéoblastes (Par similarité). Participe à la régulation du dépôt de collagène de type I par les ostéoblastes (par similarité). (UniProt)

  • Indications d'application

    Immunohistochemistry Dilution: 1:50 - 1:200

    Application Note: Anti-fibronectin (rabbit) antibody was assayed by immunoblot and found to be reactive against Fibronectin at a dilution of 1:5,000 to 1:10,000. This product was also assayed against 1.0 μg of Fibronectin in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:8,000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Restrictions
    For Research Use only
  • Validation #104084 (Unfolding Profile)
    'Independent Validation' signe
    by
    NanoTemper Technologies
    No.
    #104084
    Date
    23.07.2019
    Antigène
    Fibronectin
    Numéro du lot
    39102
    Application validée
    Unfolding Profile
    Contrôle positif
    ABIN5596762
    Contrôle négative
    Conclusion

    Passed. ABIN5596762 showed Ti at 63.4°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    Anticorps secondaire
    Full Protocol
    • Dilute ABIN5596762 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 30µl at a concentration of 0.5µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Notes

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    Fibronectin
    Abstract
    Fibronectin Produits
    Synonymes
    anticorps FN, anticorps cig, anticorps fibronectin, anticorps finc, anticorps lets, anticorps msf, anticorps CIG, anticorps ED-B, anticorps FINC, anticorps FNZ, anticorps GFND, anticorps GFND2, anticorps LETS, anticorps MSF, anticorps E330027I09, anticorps Fn, anticorps Fn-1, anticorps FIBNEC, anticorps fn-1, anticorps fibronectin 1, anticorps FN1, anticorps fn1, anticorps Fn1
    Sujet

    Synonyms: CIG antibody, Cold insoluble globulin antibody, LETS antibody, Migration stimulating factor antibody, MSF antibody, Transformation sensitive protein. Antibody

    Background: Fibronectin antibody reacts with human fibronectin in liver, tonsil, skin and kidney. Traces of contaminating antibodies have been removed by solid-phase absorption. Biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptavidin, an attraction that is the strongest and most stable non-covalent interaction known. Fibronectin is found in two forms in vertebrates: soluble and insoluble. Soluble plasma fibronectin is contained in blood plasma and constitutes a large protein component. Insoluble cellular fibronectin is a large component of the extra-cellular matrix where it is secreted by many different types of cells. Fibronectin plays a large role in wound healing and cell development. Anti-fibronectin (rabbit) antibody is ideal for investigators in Cardiology, Cell Biology, Microbiology, and Immunology research.

    Gene Name: FN1

    ID gène
    2335
    UniProt
    P02751
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