Cholesterol Oxidase anticorps (HRP)

N° du produit ABIN5596764

Détail du produit anti-Cholesterol Oxidase anticorps (HRP)

Antigène: Cholesterol Oxidase (choS)

Reactivité: Streptomyces

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Cholesterol Oxidase est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Réactivité croisée (Details): Cross reactivity against Cholesterol Oxidase from other sources is unknown.

Purification: Cholesterol Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Cholesterol Oxidase [Microorganism].

Immunogène:

Immunogen: Cholesterol Oxidase [Streptomyces]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Cholesterol Oxidase has been assayed against 1.0 μg of Cholesterol Oxidase [Microorganism] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Cholesterol Oxidase

Antigène: Cholesterol Oxidase (choS)

Autre désignation: Cholesterol Oxidase (choS Produits)

Sujet:

Synonyms: choA antibody, choD antibody, choM antibody, choS antibody, COD antibody

Background: Cholesterol Oxidase is a bifunctional enzyme that catalyzes the oxidation of the 3-beta-hydroxy group of cholesterol and the isomerization of the double bond of the resulting product. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

Gene Name: choA

ID gène: 1215660

UniProt: P12676