SLC2A2 anticorps (C-Term)

N° du produit ABIN5596789

Détail du produit anti-SLC2A2 anticorps

Antigène: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Épitope: C-Term

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SLC2A2 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Glut2 from mouse and rat based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Purification: Glut-2 antibody is directed against Glut2 protein. The product is delipidated and defibrinated antiserum.

Immunogène:

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminal domain of mouse Glut2 protein.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 4 μg/mL

Application Note: Glut-2 antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 57.1 kDa in size corresponding to Glut2 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.

Western Blot Dilution: 1:500-1:1000

ELISA Dilution: 1:30,000-1:90:000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 80 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-SLC2A2 (ABIN5596789)

Chhabra, Adams, Fagel, Lam, Qi, Rubinstein, Low: "Hypothalamic POMC Deficiency Improves Glucose Tolerance Despite Insulin Resistance by Increasing Glycosuria." dans: Diabetes, Vol. 65, Issue 3, pp. 660-72, (2016) (PubMed).

Détails sur SLC2A2

Antigène: SLC2A2 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 2 (SLC2A2))

Autre désignation: Glut2 (SLC2A2 Produits)

Synonymes: anticorps glut2, anticorps wu:fb62h10, anticorps slc2a2, anticorps MGC83262, anticorps SLC2A2, anticorps GLUT2, anticorps GTT2, anticorps Glut2, anticorps AI266973, anticorps Glut-2, anticorps solute carrier family 2 (facilitated glucose transporter), member 2, anticorps solute carrier family 2 member 2 L homeolog, anticorps solute carrier family 2 member 2, anticorps solute carrier family 2, facilitated glucose transporter member 2, anticorps slc2a2, anticorps slc2a2.L, anticorps SLC2A2, anticorps LOC100534484, anticorps Slc2a2

Sujet:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 2, Glucose transporter type 2, liver-GLUT-2, Slc2a2, Glut2 Antibody

Background: The Anti-Glut2 antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The glucose transporter GLUT2 is a transmembrane carrier protein that allows protein facilitated glucose movement across cell membranes. GLUT2 is expressed in the plasma membranes of the liver, intestine, renal tubular cells, pancreatic islet beta cells, as well as in the portal and hypothalamic areas. Due to its low affinity and high capacity, GLUT2 transports dietary sugars, glucose, galactose and fructose in high concentrations, displaying large bidirectional fluxes in and out of cells. In pancreatic beta cells, GLUT2 is essential for glucose-stimulated insulin secretion. GLUT2 expression is necessary for the physiological control of glucose-sensitive genes, and its inactivation in the liver leads to impaired glucose-stimulated insulin secretion. In the nervous system, GLUT2-dependent glucose sensing regulates feeding, thermoregulation and pancreatic islet cell mass and function, as well as sympathetic and parasympathetic activities. In humans, inactivating mutations in GLUT2 cause Fanconi-Bickel syndrome, which is characterized by hepatomegaly and kidney disease. Anti-Glut2 is ideal for researchers interested in studying glucose transport mediated by Glut2 protein in the fields of diabetes, obesity, metabolism, and neuroscience research.

Gene Name: Slc2a2

ID gène: 20526

UniProt: P14246

Pathways: L'effet Warburg