Collagen Type I anticorps (FITC)

N° du produit ABIN5596822

Détail du produit anti-Collagen Type I anticorps (FITC)

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Mammifères

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Collagen Type I est conjugé à/à la FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Réactivité croisée (Details): Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has negligible cross-reactivity with Type II, III, IV, V and VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Purification: This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

Immunogène:

Immunogen: Collagen Type I from human and bovine placenta.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 2-7

Information d'application

Indications d'application: Anti-COLLAGEN Type I Fluorescein Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL to 20 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Date de péremption: 12 months

Références pour anticorps anti-Collagen Type I (FITC) (ABIN5596822)

Liu, Pan, Cao, Shu, Sun, Lu, Liang, Zhang, Zhu, Li, Zhang: "IL-32γ promotes integrin αvβ6 expression through the activation of NF-κB in HSCs." dans: Experimental and therapeutic medicine, Vol. 14, Issue 4, pp. 3880-3886, (2017) (PubMed).

Détails sur Collagen Type I

Antigène: Collagen Type I (COL1) (Collagen, Type I (COL1))

Autre désignation: COLLAGEN Type I (COL1 Produits)

Synonymes: anticorps collagen type III alpha 1 chain, anticorps COL3A1

Sujet:

Synonyms: Collagen Of Skin Tendon And Bone antibody, Collagen Type 1 antibody, Collagen type I alpha 1 antibody, Collagen type I alpha 2 antibody, OI4 antibody, Osteogenesis Imperfecta Type I antibody

Background: COLLAGEN Type I Fluorescein Conjugated Antibody is specific for Collagen Type I. Collagen Type I (Type-I collagen) is the most abundant collagen of the human body. It is present in scar tissue, the end product when tissue heals by repair. It is also found in tendons, the endomysium of myofibrils and the organic part of bone. Anti-collagen Type I antibody is suitable for Cancer research and other general research.

Gene Name: COL1A1

ID gène: 1277

UniProt: P02452