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COL2 anticorps

COL2 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Mammifères, Mouton WB, ELISA, IHC, IP Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN5596824
  • Antigène Voir toutes COL2 Anticorps
    COL2 (Collagen, Type II (COL2))
    Reactivité
    • 44
    • 32
    • 28
    • 23
    • 18
    • 12
    • 11
    • 3
    Humain, Souris, Rat, Boeuf (Vache), Mammifères, Mouton
    Hôte
    • 41
    • 13
    • 10
    • 4
    Lapin
    Clonalité
    • 55
    • 13
    Polyclonal
    Conjugué
    • 34
    • 12
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp COL2 est non-conjugé
    Application
    • 50
    • 37
    • 21
    • 21
    • 16
    • 14
    • 13
    • 13
    • 9
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
    Réactivité croisée (Details)
    Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
    Purification
    Collagen II Antibody has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
    Immunogène

    Immunogen: Collagen Type II from human knee cartilage and bovine nasal cartilage.

    Immunogen Type: Native Protein

    Isotype
    IgG
    Top Product
    Discover our top product COL2 Anticorps primaire
    Informations sur le produit

    À quoi peut servir l'anticorps COL2 ABIN5596824 ? Cet anticorps polyclonal non conjugué de lapin COL2 convient à la détection du collagène de type 2 (COL2) par ELISA, immunohistochimie, immuniprécipitation et Western blotting. L'anticorps reconnaît COL2 chez l'homme, la souris, le rat, le bovin, le mouton et d'autres mammifères.

    Quelles sont les données de validation disponibles pour cet anticorps COL2 ? Le produit a été validé indépendamment par NanoTemper Technologies en déterminant le profil de dépliage. Il y a actuellement 2 images de produit disponibles montrant les performances de l'anticorps COL2. Utilisez notre anticorps pour détecter COL2 de manière fiable.

    Quelle est la fonction de COL2 ? Le collagène de type 2 (COL2) est spécifique des tissus cartilagineux. Il est essentiel pour le développement embryonnaire normal du squelette, pour la croissance linéaire et pour la capacité du cartilage à résister aux forces de compression. L'anticorps COL2 a pour fonction de détecter le collagène de type 2.

  • Indications d'application

    Immunohistochemistry Dilution: 1:50 - 1:400

    Application Note: Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, immunohistochemistry, native (non-denaturing, non-dissociating) PAGE, and western blotting for highly sensitive qualitative analysis.

    Western Blot Dilution: 1:1,000 - 1:10,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:50,000

    Restrictions
    For Research Use only
  • Validation #104083 (Unfolding Profile)
    'Independent Validation' signe
    by
    NanoTemper Technologies
    No.
    #104083
    Date
    23.07.2019
    Antigène
    COL2
    Numéro du lot
    41639
    Application validée
    Unfolding Profile
    Contrôle positif
    ABIN5596824
    Contrôle négative
    Conclusion

    Passed. ABIN5596824 showed Ti at 78.9°C and a clear unfolding profile with one unfolding event. This suggests that the antibody is properly folded and functional.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    Anticorps secondaire
    Full Protocol
    • Dilute ABIN5596824 in PBS buffer (Roth, 1058.1, Lot 285231988) to get a final volume of 30µl at a concentration of 0.1µM.
    • Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
    • Run Tycho measurement.
    Notes

    Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (Ti). The Ti can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of Ti would suggest that the protein is already unfolded and therefore most likely nonfunctional.

  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

    Stabilizer: None

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène
    COL2 (Collagen, Type II (COL2))
    Autre désignation
    Collagen Type II (COL2 Produits)
    Synonymes
    anticorps PT, anticorps RPRGL2, anticorps THPH1, anticorps Cf-2, anticorps Cf2, anticorps FII, anticorps thrombin, anticorps prothrombin, anticorps CAP18, anticorps Cnlp, anticorps Cramp, anticorps FALL39, anticorps MCLP, anticorps coagulation factor II, thrombin, anticorps coagulation factor II, anticorps cathelicidin antimicrobial peptide, anticorps Cuticle collagen 2, anticorps collagen type II alpha 1 chain, anticorps F2, anticorps Camp, anticorps col-2, anticorps COL2A1
    Sujet

    Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody

    Background: Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ''Glycine-X-Y'' triplet repeat that is a necessary part of the triple helical structure. For these reasons, it is often extremely difficult to generate antibodies with specificities to collagens. The development of 'type' specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. We extensively purify collagens for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions are used for SDS-PAGE and immunoblotting. Ideal for investigators involved in Cell Biology, Signal Transduction and Stem Cell research.

    Gene Name: COL2A1

    ID gène
    1280
    UniProt
    P02458
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