COL2 anticorps (HRP)
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- Antigène Voir toutes COL2 Anticorps
- COL2 (Collagen, Type II (COL2))
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Reactivité
- Humain, Boeuf (Vache), Mammifères
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp COL2 est conjugé à/à la HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)
- Réactivité croisée (Details)
- Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- Purification
- Anti-Collagen Type II has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.
- Immunogène
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Immunogen: Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product COL2 Anticorps primaire
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- Indications d'application
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Immunohistochemistry Dilution: 1:200 - 1:1,000
Application Note: Anti-Collagen Type II Peroxidase Conjugated Antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below.
Western Blot Dilution: 1:5,000 - 1:10,000
Immunoprecipitation Dilution: 1:100
ELISA Dilution: 1:10,000 - 1:50,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL to 20 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Agent conservateur
- Thimerosal (Merthiolate)
- Précaution d'utilisation
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
- Date de péremption
- 12 months
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- Antigène
- COL2 (Collagen, Type II (COL2))
- Autre désignation
- Collagen Type II (COL2 Produits)
- Synonymes
- anticorps PT, anticorps RPRGL2, anticorps THPH1, anticorps Cf-2, anticorps Cf2, anticorps FII, anticorps thrombin, anticorps prothrombin, anticorps CAP18, anticorps Cnlp, anticorps Cramp, anticorps FALL39, anticorps MCLP, anticorps coagulation factor II, thrombin, anticorps coagulation factor II, anticorps cathelicidin antimicrobial peptide, anticorps Cuticle collagen 2, anticorps collagen type II alpha 1 chain, anticorps F2, anticorps Camp, anticorps col-2, anticorps COL2A1
- Sujet
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Synonyms: Cartilage collagen antibody, Chondrocalcin antibody, COL11A3 antibody, COL2A1 antibody
Background: Anti-Collagen Type II antibody detects Type-II collagen which is the basis for articular cartilage and hyaline cartilage. It makes up 50 % of all protein in cartilage and 85-90 % of collagen of articular cartilage. Type II collagen does form fibrils which allow cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue. Anti-Collagen Type II antibody is ideal for investigators involved in Immunology, Neuroscience and Signal Transduction research.
Gene Name: COL2A1
- ID gène
- 1280
- UniProt
- P02458
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