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AKT3 anticorps (Internal Region) (APC)

AKT3 Reactivité: Humain, Souris, Rat WB, ELISA, IHC, FACS, FM Hôte: Souris Monoclonal 25F6-F6-D8 APC
N° du produit ABIN5596894
  • Antigène Voir toutes AKT3 Anticorps
    AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
    Épitope
    • 14
    • 11
    • 10
    • 8
    • 6
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivité
    • 107
    • 58
    • 42
    • 11
    • 10
    • 9
    • 6
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Hôte
    • 92
    • 24
    • 1
    Souris
    Clonalité
    • 83
    • 34
    Monoclonal
    Conjugué
    • 57
    • 10
    • 8
    • 8
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp AKT3 est conjugé à/à la APC
    Application
    • 94
    • 57
    • 39
    • 12
    • 9
    • 7
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Réactivité croisée (Details)
    Cross reactivity with AKT3 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT has not been determined.
    Purification
    Anti-AKT3 antibody is directed against human AKT3. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT3 antibody was purified from ascites by Protein A chromatography.
    Immunogène

    Immunogen: Anti-AKT3 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT3 protein.

    Immunogen Type: Peptide

    Clone
    25F6-F6-D8
    Isotype
    IgG1
    Labeling Ratio
    1-2
    Top Product
    Discover our top product AKT3 Anticorps primaire
  • Indications d'application

    Flow Cytometry Dilution: 1.0 μg/mL

    Immunohistochemistry Dilution: User optimized

    Application Note: Anti-AKT3 APC Antibody is suitable for Flow Cytometry, ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT3 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

    Western Blot Dilution: User Optimized

    ELISA Dilution: User Optimized

    IF Microscopy Dilution: User optimized

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 100 μL to 20 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Agent conservateur
    Without preservative
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
    Date de péremption
    12 months
  • Antigène
    AKT3 (V-Akt Murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, Gamma) (AKT3))
    Autre désignation
    AKT3 (AKT3 Produits)
    Synonymes
    anticorps MPPH, anticorps PKB-GAMMA, anticorps PKBG, anticorps PRKBG, anticorps RAC-PK-gamma, anticorps RAC-gamma, anticorps STK-2, anticorps AI851531, anticorps D930002M15Rik, anticorps Nmf350, anticorps Pkbg, anticorps AKT serine/threonine kinase 3, anticorps thymoma viral proto-oncogene 3, anticorps v-akt murine thymoma viral oncogene homolog 3b, anticorps AKT3, anticorps Akt3, anticorps akt3b
    Sujet

    Synonyms: AKT 3 antibody, AKT-3, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 3 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma

    Background: AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT3 (MOUSE) APC conjugated Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

    Gene Name: AKT3

    ID gène
    10000
    UniProt
    Q9Y243
    Pathways
    Signalisation PI3K-Akt, Signalisation RTK, Signalisation TLR, Hepatitis C, VEGF Signaling
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