Sucrose Phosphorylase anticorps (HRP)

N° du produit ABIN5596915

Détail du produit anti-Sucrose Phosphorylase anticorps (HRP)

Antigène: Sucrose Phosphorylase

Reactivité: E. coli

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Sucrose Phosphorylase est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Réactivité croisée (Details): Cross reactivity against Sucrose Phosphorylase from other sources is unknown.

Purification: Sucrose Phosphorylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Sucrose Phosphorylase [E.coli].

Immunogène:

Immunogen: Sucrose Phosphorylase [E.coli]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Sucrose Phosphorylase has been assayed against 1.0 μg of Sucrose Phosphorylase [E.coli] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur Sucrose Phosphorylase

Antigène: Sucrose Phosphorylase

Abstract: Sucrose Phosphorylase Produits

Synonymes: anticorps sucrose phosphorylase, anticorps Sucrose phosphorylase, anticorps RB12343, anticorps Shewmr4_0064, anticorps Shewmr7_0062, anticorps Sfri_3990, anticorps Acid_2070, anticorps Shewana3_0066, anticorps BAD_RS00415, anticorps Pnap_0232, anticorps Shew185_0061, anticorps Blon_0128, anticorps Lm4b_00279, anticorps Apre_0241, anticorps EAMY_RS33260, anticorps Spirs_0911, anticorps SpiBuddy_2458, anticorps Lbuc_1855

Sujet:

Synonyms: Putative sucrose phosphorylase Sucrose glucosyltransferase

Background: Sucrose Phosphorylase is an important enzyme in the metabolism of sucrose and regulation of other metabolic intermediates. Sucrose phosphorylase is in the class of hexosyltransferases. It has been shown in multiple experiments that the enzyme catalyzes this conversion by a double displacement mechanism.

Gene Name: ycjM

ID gène: 4492194

UniProt: P76041