Superoxide Dismutase anticorps (HRP)
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- Antigène
- Superoxide Dismutase
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Reactivité
- Boeuf (Vache)
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- HRP
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Application
- Western Blotting (WB), ELISA
- Réactivité croisée (Details)
- Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
- Purification
- Superoxide Dismutase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes].
- Immunogène
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Immunogen: Superoxide Dismutase [Bovine erythrocytes]
Immunogen Type: Native Protein
- Isotype
- IgG
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- Indications d'application
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Application Note: Anti-Superoxide Dismutase has been assayed against 1.0 μg of Superoxide Dismutase [Bovine erythrocytes] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500- 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Agent conservateur
- Gentamicin sulfate
- Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- Superoxide Dismutase
- Synonymes
- anticorps GTP-binding nuclear protein Ran, anticorps superoxide dismutase 1, anticorps LOC786258, anticorps SOD1
- Sujet
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Synonyms: ALS 1 antibody, ALS antibody, ALS1 antibody, Amyotrophic lateral sclerosis 1 adult antibody, Amyotrophic lateral sclerosis 1 antibody, Cu/Zn SOD antibody, Cu/Zn superoxide dismutase antibody, Homodimer antibody
Background: Superoxide Dismutase destroys radicals which are normally produced within the cells and which are toxic to biological systems. They are an important antioxidant defense in nearly all cells exposed to oxygen. Mutations in the Superoxide Dismutase enzyme can cause ALS, A4V, G93A, Down Syndrome, ARDS, and COPD.
Gene Name: SOD1
- ID gène
- 281495
- UniProt
- P00442
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