POLA1 anticorps (AA 1-33)

N° du produit ABIN5646962

Détail du produit anti-POLA1 anticorps

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Épitope: AA 1-33

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLA1 est non-conjugé

Application: Western Blotting (WB)

Réactivité croisée (Details): Expected species reactivity: Mouse,Rat

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 1-33 from human POLA1 was used as the immunogen for the DNA Polymerase alpha antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: The stated application concentrations are suggested starting points. Titration of the DNA Polymerase alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 1:1000-1:2000

Restrictions: For Research Use only

Stockage

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the DNA Polymerase alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur POLA1

Antigène: POLA1 (DNA Polymerase alpha (POLA1))

Autre désignation: DNA Polymerase alpha / POLA1 (POLA1 Produits)

Synonymes: anticorps pola, anticorps AW321876, anticorps Pola, anticorps POLA, anticorps p180, anticorps polymerase (DNA directed), alpha 1, catalytic subunit S homeolog, anticorps polymerase (DNA directed), alpha 1, anticorps DNA polymerase alpha 1, catalytic subunit, anticorps pola1.S, anticorps Pola1, anticorps POLA1

Sujet: Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

UniProt: P09884

Pathways: SARS-CoV-2 Protein Interactome