Lapin anti-Singe IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed

N° du produit ABIN5706887

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Singe

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: FITC

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Réactivité croisée: Singe

Attributs du produit:

Synonyms: rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody

Background: Anti-Monkey IgG (H&L) FITC antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This fluorescence conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Purification: Preadsorption: immunoaffinity chromatography using Monkey IgG coupled to agarose beads

Immunogène:

Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.

Immunogen Type: Native Protein

Isotype: IgG

Labeling Ratio: 4.6

Information d'application

Indications d'application:

Application Note: Anti-Monkey IgG (H&L) FITC conjugated Antibody is suitable for use in immunoelectrophoresis, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Specific conditions for reactivity and signal detection should be optimized by the end user.

FLISA Dilution: 1:50,000 - 1:100,000

Flow Cytometry Dilution: User Optimized

Western Blot Dilution: 1:10,000 - 1:60,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: rabbit Anti-Monkey IgG peroxidase Conjugated Antibody, rabbit Anti-Monkey IgG Antibody peroxidase Conjugation, rabbit Anti-Monkey IgG HRP Conjugated Antibody

Background: Anti-Monkey IgG (H&L) FITC antibody generated in rabbit detects specifically monkey IgG heavy and light chains. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This fluorescence conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.