SPRY1 anticorps (C-Term)

N° du produit ABIN570913

Détail du produit anti-SPRY1 anticorps

Antigène: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Épitope: C-Term

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp SPRY1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Fonction: Sprouty

Séquence: CPSRGQGKPS

Specificité: NP_005832.1 and NP_955359.1 represent varients of the same protein.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Classe de qualité: Verified

Immunogène: Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.

Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS

Peptide ELISA: antibody detection limit dilution 1:16000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur SPRY1

Antigène: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Autre désignation: SPRY1 (SPRY1 Produits)

Synonymes: anticorps hSPRY1, anticorps SPRY1, anticorps LOC100223875, anticorps hspry1, anticorps xspry1, anticorps sprouty, anticorps sprouty1, anticorps sprouty-1, anticorps Xtsprouty1, anticorps spry1a, anticorps spry1b, anticorps sprouty RTK signaling antagonist 1, anticorps sprouty RTK signaling antagonist 1 L homeolog, anticorps sprouty RTK signaling antagonist 1 S homeolog, anticorps sprouty homolog 1 (Drosophila), anticorps SPRY1, anticorps Spry1, anticorps spry1, anticorps spry1.L, anticorps spry1.S

Sujet: SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)

ID gène: 10252

NCBI Accession: NP_005832, NP_955359

Pathways: EGFR Signaling Pathway, EGFR Downregulation