DICER1 anticorps

N° du produit ABIN577054

Détail du produit anti-DICER1 anticorps

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Reactivité: Humain, Souris, Rat, Poisson zèbre (Danio rerio), Lézard

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DICER1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Specificité: Greater than 95%

Purification: Affinity purified

Pureté: > 98 %

Immunogène: Synthetic Peptide

Isotype: IgG

Information d'application

Indications d'application: Works on IHC at 1/10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1mg/ml

Buffer: PBS/glycerol

Stock: -20 °C

Références pour anticorps anti-DICER1 (ABIN577054)

Wang, McAllister, Bailey, Scott, Hendley, Leach, Ghosh: "Dicer is required for maintenance of adult pancreatic acinar cell identity and plays a role in Kras-driven pancreatic neoplasia." dans: PLoS ONE, Vol. 9, Issue 11, pp. e113127, (2014) (PubMed).

Caffrey, Ingoldsby, Wall, Webber, Dinneen, Murillo, Inderhaug, Newell, Gupta, Callagy: "Prognostic significance of deregulated dicer expression in breast cancer." dans: PLoS ONE, Vol. 8, Issue 12, pp. e83724, (2014) (PubMed).

Jafarnejad, Sjoestroem, Ardekani, Martinka, Li: "Reduced expression of nuclear Dicer correlates with melanoma progression." dans: Pigment cell & melanoma research, Vol. 26, Issue 1, pp. 147-9, (2012) (PubMed).

Détails sur DICER1

Antigène: DICER1 (Dicer 1, Ribonuclease Type III (DICER1))

Autre désignation: Dicer (DICER1 Produits)

Synonymes: anticorps dcr1, anticorps dicer, anticorps herna, anticorps DICER1, anticorps dicer1, anticorps 1110006F08Rik, anticorps D12Ertd7e, anticorps mKIAA0928, anticorps DCR1, anticorps Dicer, anticorps HERNA, anticorps MNG1, anticorps DICER, anticorps fc39d11, anticorps fc74b05, anticorps wu:fc39d11, anticorps wu:fc74b05, anticorps dicer 1, ribonuclease III, anticorps endoribonuclease Dicer, anticorps dicer 1, ribonuclease III S homeolog, anticorps dicer 1, ribonuclease type III, anticorps dicer 1 ribonuclease III, anticorps DICER1, anticorps CpipJ_CPIJ010534, anticorps dicer1, anticorps dicer1.S, anticorps Dicer1

Sujet: High affinity rabbit derivative of 13D6. Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides long, usually with a two-base overhang on the 3' end. Dicer contains two RNase III domains and one PAZ domain, the distance between these two regions of the molecule is determined by the length and angle of the connector helix and determines the length of the siRNAs it produces. Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand. The human version of this gene is DICER1. In recent work, CLONEGENE has developed a rabbit antibody (R13D6) against a highly conserved peptide in human Dicer. This antibody reacts with human, mouse, rat, cow, dog and lizard Dicer . The sequence of the peptide is confidential but we are happy to advise you if this antibody will work on Dicer from your species of interest. Working conditions are 1/20,000 for IHC and 1/5000 for western blot making this a very economical purchase. R13D6 was generated against the same synthetic peptide that was used to generate our anti-Dicer 13D6 and 4A6 mouse monoclonals. Indeed R12D6 is superior to 4A6 in IHC on archival paraffin sections (see image of R13D6 on FFPE lung tumor section ). R13D6 is provided as an affinity-purified IgG (1mg/ml) .

Pathways: Regulatory RNA Pathways, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization