Souris anti-Humain IgM Anticorps
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- Antigène Tous les produits IgM
- IgM
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Reactivité
- Humain
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Hôte
- Souris
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Clonalité
- Monoclonal
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Fonction
- Mouse Monoclonal anti-Human IgM Immunoglobulin (IM260), Purified, BSA-free
- Attributs du produit
- This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Purification
- Purified
- Immunogène
- Recombinant heavy chain of human IgM
- Clone
- IM260
- Isotype
- IgG1 kappa
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- Indications d'application
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Immunohistology formalin-fixed 0.5-1 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Commentaires
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293T, Raji or hPBL cells. Tonsil or Spleen
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS (no BSA, no azide)
- Agent conservateur
- Azide free
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- Antigène
- IgM
- Autre désignation
- IgM Immunoglobulin (IgM Produits)
- Classe de substances
- Antibody
- Sujet
- AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region
- Poids moléculaire
- 50-75 kDa
- ID gène
- 3507, 510635
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