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Souris anti-Humain IgM Anticorps

FACS, WB, IF, IHC (f) Monoclonal IM260 IgG1 kappa unconjugated
N° du produit ABIN6155086
  • Antigène Tous les produits IgM
    IgM
    Reactivité
    • 192
    • 134
    • 66
    • 39
    • 32
    • 26
    • 22
    • 21
    • 16
    • 15
    • 15
    • 14
    • 13
    • 13
    • 12
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 321
    • 188
    • 78
    • 19
    • 14
    • 5
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 496
    • 87
    Monoclonal
    Application
    • 336
    • 250
    • 241
    • 210
    • 143
    • 139
    • 64
    • 63
    • 47
    • 47
    • 47
    • 42
    • 38
    • 29
    • 20
    • 18
    • 13
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Fonction
    Mouse Monoclonal anti-Human IgM Immunoglobulin (IM260), Purified, BSA-free
    Attributs du produit
    This antibody recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Purification
    Purified
    Immunogène
    Recombinant heavy chain of human IgM
    Clone
    IM260
    Isotype
    IgG1 kappa
  • Indications d'application
    Immunohistology formalin-fixed 0.5-1 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Immunofluorescence 0.5-1 μg/mL
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Commentaires

    293T, Raji or hPBL cells. Tonsil or Spleen

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS (no BSA, no azide)
    Agent conservateur
    Azide free
  • Antigène
    IgM
    Autre désignation
    IgM Immunoglobulin (IgM Produits)
    Classe de substances
    Antibody
    Sujet
    AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region
    Poids moléculaire
    50-75 kDa
    ID gène
    3507, 510635
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