TAG-72 anticorps

N° du produit ABIN6252881

Détail du produit anti-TAG-72 anticorps

Antigène: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Reactivité: Humain, Rat, Boeuf (Vache), Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TAG-72 est non-conjugé

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Protein G affinity chromatography

Immunogène: Purified TAG-72 protein was used as the immunogen for this TAG-72 antibody.

Clone: CC49

Isotype: IgG1

Information d'application

Indications d'application: Western blot: 1-2 μg/mL

Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the TAG-72 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Détails sur TAG-72

Antigène: TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))

Autre désignation: TAG-72 (TAG-72 Produits)

Synonymes: anticorps mRNA cap guanine-N7 methyltransferase, anticorps tag-72

Sujet: Recognizes an oncofetal antigen of 220  kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma.