MAPK14 anticorps (pTyr323)

N° du produit ABIN6256707

Détail du produit anti-MAPK14 anticorps

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Épitope: pTyr323

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MAPK14 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Phospho-p38 MAPK (Tyr323) Antibody detects endogenous levels of p38 MAPK only when phosphorylated at Tyrosine 323.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human p38 MAPK around the phosphorylation site of Tyr323.

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur MAPK14

Antigène: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Autre désignation: MAPK14 (MAPK14 Produits)

Synonymes: anticorps CSBP, anticorps CSBP1, anticorps CSBP2, anticorps CSPB1, anticorps EXIP, anticorps Mxi2, anticorps PRKM14, anticorps PRKM15, anticorps RK, anticorps SAPK2A, anticorps p38, anticorps p38ALPHA, anticorps CRK1, anticorps Csbp1, anticorps Csbp2, anticorps Exip, anticorps Hog, anticorps Prkm14, anticorps Prkm15, anticorps Sapk2A, anticorps p38Hog, anticorps p38alpha, anticorps p38b, anticorps zp38b, anticorps MAPK14, anticorps 186F5S, anticorps BG:DS00797.3, anticorps CG7393, anticorps D-p38, anticorps D-p38 MAPK, anticorps D-p38b, anticorps Dm p38b, anticorps Dmel\\CG7393, anticorps Dmp38b, anticorps Dp38, anticorps Dp38b, anticorps ESTS:186F5S, anticorps Mpk34C, anticorps anon-sts23, anticorps dp38b, anticorps p38 MAPK, anticorps p38 beta, anticorps p38B, anticorps p38Kb, anticorps p38beta, anticorps Crk1, anticorps p38-alpha, anticorps p38MAPK, anticorps p38a, anticorps csbp, anticorps mapk14a, anticorps mxi2, anticorps sapk2, anticorps sapk2a, anticorps AP22.98, anticorps AP22_98, anticorps ATMPK14, anticorps mitogen-activated protein kinase 14, anticorps SAPK2a, anticorps MAP kinase 14A, anticorps MAP kinase p38a, anticorps MAPK 14A, anticorps fk28c03, anticorps hm:zeh1243, anticorps wu:fk28c03, anticorps zp38a, anticorps P38C-CRK, anticorps mitogen-activated protein kinase 14, anticorps mitogen activated protein kinase 14, anticorps mitogen-activated protein kinase 14b, anticorps p38b MAP kinase, anticorps mitogen-activated protein kinase 14 S homeolog, anticorps mitogen-activated protein kinase 14a, anticorps CRK proto-oncogene, adaptor protein, anticorps MAPK14, anticorps Mapk14, anticorps mapk14b, anticorps p38b, anticorps mapk14.S, anticorps MPK14, anticorps mapk14a, anticorps CRK

Sujet:

Description: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated through phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1, ATF1, the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1, and by regulating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, regulate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation through autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important regulator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is regulated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to regulate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which results in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracellular space into the cytosol and nucleus of target cells, and regulates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after ultraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are thought to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is regulated by MAPK14, and, although OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may regulate OGT activity by recruiting it to specific targets such as neurofilament H, stimulating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at 'Thr-113'.

Gene: MAPK14

Poids moléculaire: 43kDa

ID gène: 1432

UniProt: Q16539

Pathways: Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins