GBL anticorps
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- Antigène Voir toutes GBL Anticorps
- GBL (G protein beta subunit-like (GBL))
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Reactivité
- Humain, Souris, Rat
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp GBL est non-conjugé
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificité
- MLST8 Antibody detects endogenous levels of total MLST8
- Réactivité croisée
- Humain, Souris, Rat (Rattus)
- Purification
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogène
- A synthesized peptide derived from human MLST8
- Isotype
- IgG
- Top Product
- Discover our top product GBL Anticorps primaire
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- Indications d'application
- WB 1:500-1:2000 IHC 1:50-1:200
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- -20 °C
- Stockage commentaire
- Store at -20 °C.Stable for 12 months from date of receipt
- Date de péremption
- 12 months
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- Antigène
- GBL (G protein beta subunit-like (GBL))
- Autre désignation
- MLST8 (GBL Produits)
- Synonymes
- anticorps gbl, anticorps lts8, anticorps GBL, anticorps GbetaL, anticorps LST8, anticorps POP3, anticorps WAT1, anticorps 0610033N12Rik, anticorps AA409454, anticorps AI505104, anticorps AI851821, anticorps Gbl, anticorps fi37e04, anticorps wu:fi37e04, anticorps zgc:55455, anticorps zgc:85668, anticorps MTOR associated protein, LST8 homolog L homeolog, anticorps MTOR associated protein, LST8 homolog, anticorps MTOR associated protein, LST8 homolog (S. cerevisiae), anticorps mlst8.L, anticorps MLST8, anticorps Mlst8, anticorps mlst8
- Sujet
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Description: Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
Gene: MLST8
- Poids moléculaire
- 36kDa
- ID gène
- 64223
- UniProt
- Q9BVC4
- Pathways
- Signalisation PI3K-Akt, Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, L'effet Warburg
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