POLD4 anticorps (Internal Region)

N° du produit ABIN6264334

Détail du produit anti-POLD4 anticorps

Antigène: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Épitope: Internal Region

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLD4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificité: POLD4 Antibody detects endogenous levels of total POLD4.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit,Dog

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogène: A synthesized peptide derived from human POLD4, corresponding to a region within the internal amino acids.

Isotype: IgG

Information d'application

Indications d'application: WB 1:1000-3000, IHC 1:200, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C. Stable for 12 months from date of receipt.

Date de péremption: 12 months

Détails sur POLD4

Antigène: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Autre désignation: POLD4 (POLD4 Produits)

Synonymes: anticorps 2410012M21Rik, anticorps AI463381, anticorps AW060307, anticorps Polds, anticorps p12, anticorps POLDS, anticorps DNA polymerase delta 4, accessory subunit, anticorps polymerase (DNA-directed), delta 4, anticorps POLD4, anticorps Pold4

Sujet:

Description: As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity (PubMed:16510448, PubMed:19074196, PubMed:20334433). Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system (PubMed:24035200). Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196). Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480).

Gene: POLD4

Poids moléculaire: 12 kDa

ID gène: 57804

UniProt: Q9HCU8

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA