STAT1 anticorps (pSer727)

N° du produit ABIN6267508

Détail du produit anti-STAT1 anticorps

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Épitope: pSer727

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp STAT1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificité: Phospho-STAT1 (Ser727) Antibody detects endogenous levels of STAT1 only when phosphorylated at Serine 727

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Immunogène: A synthesized peptide derived from human STAT1 around the phosphorylation site of Serine 727

Isotype: IgG

Information d'application

Indications d'application: WB 1:500-1:2000 IHC 1:50-1:200, IF/ICC 1:100-1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20 °C.Stable for 12 months from date of receipt

Date de péremption: 12 months

Références pour anticorps anti-STAT1 (ABIN6267508)

Wu, Chen, Jing, Gu, Mei, Yao, Zhou, Yang, Sun, Wang, Hu, Wüthrich, Mei: "The C-terminal tail of polycystin-1 regulates complement factor B expression by signal transducer and activator of transcription 1." dans: American journal of physiology. Renal physiology, Vol. 310, Issue 11, pp. F1284-94, (2017) (PubMed).

Détails sur STAT1

Antigène: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Autre désignation: STAT1 (STAT1 Produits)

Synonymes: anticorps CANDF7, anticorps ISGF-3, anticorps STAT91, anticorps 2010005J02Rik, anticorps AA408197, anticorps XStat1, anticorps STAT4, anticorps DD6G4-4, anticorps STAT, anticorps STAT1, anticorps dZ182H3.3, anticorps si:by134g18.2, anticorps si:by51f19.1, anticorps si:dz182h3.3, anticorps si:dz199m19.1, anticorps si:dz73o4.2, anticorps stat1, anticorps signal transducer and activator of transcription 1, anticorps signal transducer and activator of transcription 1 L homeolog, anticorps signal transducer and activator of transcription 1, 91kDa, anticorps signal transducer and activator of transcription 4, anticorps signal transducer and activator of transcription 1a, anticorps STAT1, anticorps Stat1, anticorps stat1.L, anticorps stat4, anticorps stat1a

Sujet:

Description: Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus (PubMed:28753426). ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated (PubMed:26479788). It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gene: STAT1

Poids moléculaire: 91kDa

ID gène: 6772

UniProt: P42224

Pathways: Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events