Folic Acid (FA) anticorps

Détails pour le produit réf. ABIN6560025
Antigène
Reactivité
Chemical
19
1
1
1
Hôte
Rat
28
9
1
Clonalité
Polyclonal
Conjugué
Inconjugué
2
1
Application
Immunohistochemistry (IHC)
22
7
6
5
3
2
2
2
1
1
Options
Immunogène Synthetic Folic Acid conjugated to bovine serum albumin (BSA)
Isotype IgG
Specificité Folic Acid (Vitamin B9) . Using a conjugate Folic acid-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compound Cross-reactivity ratio (a) Folic acid-BSA 1 Folinic acid-BSA 1/>900 Tetrahydrofolic acid-BSA 1/>2,000 Methotrexate-BSA 1/>50,000 Riboflavin-BSA 1/>50,000 BSA 1/>50,000
Purification Antiserum previously preabsobed on protein carriers, and purified.
Antigène
Autre désignation Folic Acid
Classe de substances Chemical
Indications d'application Immunohistochemistry Perfusion protocol for Adult male monkeys (Macaca fascicularis) (weight 3-3.5kg): 1 - The animals can be deeply anaesthetized with ketamine (8mg/kg, intramuscular) and sodium thiopental (500mg/kg, intraperitoneal). 2 - Heparinized, and perfused via the ascending aorta with 300ml of cold physiologic saline (0.9% NaCl) and with the following fixative solutions:a) 500 ml of 1% paraformaldehyde in 0.1 M phosphate-buffer (PB), pH 7.2, at room temperature (two minutes). b) 2,500ml of 4% paraformaldehyde in 0.1 M PB, pH 7.2, at 4ºC (ten minutes). c) 5,000ml of cold 4% paraformaldehyde in 0.1 M PB, pH 7.2 (fifty minutes). d) 2,000ml of cold 5% sucrose in 0.1M PB, pH 7.2 (twenty minutes). d) Dissect out the brains and place in 10% glycerol and 2% dimethylsufoxide (DMSO) in 0.1M PB, pH 7.2, at 4ºC for two days, and finally keep at the same temperature in 20% of glycerol and 2% DMSO in PB until the brains will be cut on a freezing microtome. Around 50 µm-thick serial sections will be obtained, kept at 4ºC in PB (0.1 M, pH 7.2) containing 20% of glycerol and 30% of ethylene glycol, and processed for immunostaining. Example of immunohistochemical protocol 1 - In order to avoid possible interference with endogenous peroxidase, free-floating sections will be treated with distilled water containing NH 3 (20%), H 2 O 2 (30%) and NaOH (1%) for 20 min (other method is using a solution with 33% of H 2 O 2 and 66% of methanol). 2 - Then, wash the sections for 20 min in 0.15 M phosphate-buffered saline (PBS) (pH 7.2) 3 - Pre-incubate for 30 min in PBS containing 10% of normal horse serum and 0.3% of Triton X-100 (mixed solution). 4 - Incubate at room temperature (1h30min) and overnight at 4ºC in the same mixed solution containing folic acid antiserum (diluted 1/500­1/1,000 as recommended dilutions). 5 - Then, the sections will be wash in PBS (30 min). 6 - After that we will incubate for 60 min at room temperature with biotinylated anti-rat immunogammaglobulin (Vector) diluted 1/200 in PBS. 7 - Wash during 30 min with PBS. 8 - Sections will be incubated for 1h with a 1/100 diluted avidin-biotin-peroxidase complex (Vectastain) in the mixed solution. 9 - After that we will wash the sections in PBS (30 min) 10 - Wash with Tris-HCl buffer (pH 7.6) (10 min). 11 - The tissue-bound peroxidase will be developed with H 2 O 2 using 3, 3' diaminobenzidine as chromogen. 12 - Finally the sections will be rinsed with PBS and coverslipped with PBS/Glycerol (1/1). Recommended dilutions for Immunohistochemistry (1/500-1/1,000).
Restrictions For Research Use only
Format Lyophilized
Images (Fournisseur)
Image no. 1 for anti-Folic Acid (FA) antibody (ABIN6560025) Folic Acid-immunreactive fibers located in the ventro-lateral part of the pulvinar nu...
Image no. 2 for anti-Folic Acid (FA) antibody (ABIN6560025) Immunoreactive fibers containing Folic acid in the dorsal mesencephalic region of the...