Ephrin B anticorps (pTyr317)

N° du produit ABIN6655036

Détail du produit anti-Ephrin B anticorps

Antigène: Ephrin B (EphB)

Épitope: pTyr317

Reactivité: Poulet

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ephrin B est non-conjugé

Application: Western Blotting (WB), ELISA

Réactivité croisée: Souris, Rat (Rattus)

Purification: Anti-EphrinB pY317 antibody is directed against rat Ephrin. Anti-EphrinB pY317 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated EphrinB. Minimal reactivity occurs against non-phosphorylated EphrinB pY317. Reactivity is completely blocked by treatment with lambda-phosphatase. Reactivity against EphrinB occurs from mouse and rat sources. However, reactivity is also expected against the following species based on 100% sequence homology: bovine, chicken, human, mouse, Xenopus, and zebra fish.

Immunogène:

Immunogen: Anti-EphrinB pY317 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr317 of Xenopus EphrinB conjugated to KLH.

Immunogen Type: Peptide

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-EphrinB pY317 Antibody is suitable for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 46 kDa in size corresponding to EphrinB protein phosphorylated at Tyr317 in the appropriate cell lysate or extract.

ELISA Dilution: 1:10,000

Western Blot Dilution: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Détails sur Ephrin B

Antigène: Ephrin B (EphB)

Autre désignation: EphrinB (EphB Produits)

Sujet:

Synonyms: Ephrin type-B receptor 2, EPH-like kinase 5, EK5

Background: EphrinB Antibody detects rat EphrinB. recognizes EphrinB proteins which are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event. Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling. Anti-EphrinB pY317 Antibody is ideal for investigators involved in Cell Migration, Cell Division, Cell Signaling and Neuroscience.

Gene Name: EPHB2

ID gène: 396513

NCBI Accession: NP_996834

UniProt: P28693