IKBKG anticorps

N° du produit ABIN6655353

Détail du produit anti-IKBKG anticorps

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IKBKG est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Purification: Anti-IKKg Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with IKKg from Human based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-IKKg from other sources has not been determined.

Immunogène:

Immunogen: IKK gamma Antibody was produced in mice prepared by repeated immunizations with human IKKgamma (NEMO) protein at full-length.

Immunogen Type: Native Protein

Clone: 46B844

Isotype: IgG1

Information d'application

Indications d'application:

Application Note: Anti-IKKgamma antibody is suitable for use in Flow (I) and WB. Expect a band approximately 48 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Flow Cytometry Dilution: 0.1-0.5/10^6 cells

Western Blot Dilution: 2 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur IKBKG

Antigène: IKBKG (Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase gamma (IKBKG))

Autre désignation: IKK gamma (IKBKG Produits)

Synonymes: anticorps AMCBX1, anticorps FIP-3, anticorps FIP3, anticorps Fip3p, anticorps IKK-gamma, anticorps IP, anticorps IP1, anticorps IP2, anticorps IPD2, anticorps NEMO, anticorps ZC2HC9, anticorps 1110037D23Rik, anticorps AI848108, anticorps AI851264, anticorps AW124339, anticorps IKK[g], anticorps Nemo, anticorps nemo, anticorps fj33c07, anticorps wu:fj33c07, anticorps zgc:113492, anticorps MGC97885, anticorps IKBKG, anticorps IKBKG_1, anticorps IKBKG_2, anticorps CG16910, anticorps DmIKK-gamma, anticorps DmIKKgamma, anticorps Dmel\\CG16910, anticorps Dmikkgamma, anticorps IKK, anticorps IKK[[gamma]], anticorps IKKg, anticorps IKKgamma, anticorps KEY, anticorps Kenny, anticorps Key, anticorps dIKK, anticorps dIKK-gamma, anticorps dmIKKgamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma, anticorps inhibitor of kappaB kinase gamma, anticorps inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase gamma, anticorps inhibitor of nuclear factor kappa B kinase subunit gamma L homeolog, anticorps kenny, anticorps IKBKG, anticorps Ikbkg, anticorps ikbkg, anticorps ikbkg.L, anticorps IKBKG_1, anticorps key

Sujet:

Synonyms: FIP3, NEMO, NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier

Background: Anti-IKK gamma detects human IKKgamma. NF-kappaB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kappaB thereby preventing translocation of NF-kB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKKalpha, IKKbeta, and IKKgamma (NEMO). NEMO (NF-kB Essential MOdulator) preferentially interacts with IKK2/b and is required for activation of IKK complex. Recent data suggest that the human T-cell leukemia virus type I Tax oncoprotein that activates NF-kB binds neither to IKKalpha nor IKKbeta, but complexes directly with NEMO. This suggests that NEMO may be a key molecule acting as an adapter for onco-protein specific signaling to IKKa and IKKb. Anti-IKKgamma antibody is ideal for investigators involved in NFkappaB, kinase and growth factor research.

Gene Name: IKBKG

ID gène: 8517

NCBI Accession: NP_001093326

UniProt: Q9Y6K9

Pathways: Signalisation NF-kappaB, Signalisation RTK, TCR Signaling, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, M Phase, Production of Molecular Mediator of Immune Response, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins