KCNMB3 anticorps (N-Term)

N° du produit ABIN6657686

Détail du produit anti-KCNMB3 anticorps

Antigène: KCNMB3 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3))

Épitope: N-Term

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KCNMB3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Anti-BK channel BETA3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with BK channel BETA3 from human, mouse, and rat based on 100% homology with the immunizing sequence. No cross-reactivity against BKBeta1, BKBeta1, BKBeta3b or BKBeta4. Cross-reactivity with BK channel BETA3 from other sources has not been determined. Ion Channels research.

Immunogène:

Immunogen: BK channel BETA3 Antibody was produced in mice by repeated immunizations with a fusion protein (N-terminus region) of mouse BKBeta3.

Immunogen Type: Recombinant Protein

Clone: S40B-18

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 0.1-1.0 μg/mL

Application Note: Anti-BK channel Beta3 Antibody is suitable for use in WB, IP, and IHC. Expect a band approximately ~32 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

Western Blot Dilution: 1-10 μg/mL

IF Microscopy Dilution: 1.0-10 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Détails sur KCNMB3

Antigène: KCNMB3 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M beta Member 3 (KCNMB3))

Autre désignation: BK channel BETA3 (KCNMB3 Produits)

Sujet:

Synonyms: HBETA3, KCNMB2, KCNMBL, BKBeta3, KCNMB3, Calcium-activated potassium channel subunit beta-3, BK channel subunit beta-3, BKbeta3, Hbeta3, Calcium-activated potassium channel, subfamily M subunit beta-3 Charybdotoxin receptor subunit beta-3, K(VCA)beta-3, Maxi K channel subunit beta-3, Slo-beta-3

Background: BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release. A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia. This has major implications on other physiological processes in tissues other than the brain.

Gene Name: KCNMB3

ID gène: 27094

NCBI Accession: NP_055222

UniProt: Q9NPA1