Fibrillarin anticorps

N° du produit ABIN6658299

Détail du produit anti-Fibrillarin anticorps

Antigène: Fibrillarin (FBL)

Reactivité: Saccharomyces cerevisiae

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Fibrillarin est non-conjugé

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Réactivité croisée: Humain, Souris, Rat (Rattus)

Purification: Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are protein G purified from concentrated clarified cell culture supernate. Reactivity is expected against human, mouse, and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species.

Immunogène:

Immunogen: Anti-Fibrillarin Monoclonal Antibody was produced in mouse by repeated immunizations with native yeast nuclear protein preparations.

Immunogen Type: Native Protein

Clone: 38F3

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Fibrillarin (Mouse) Antibody is suitable for use in Western Blotting and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band size of approximately 34 kDa in size corresponding to the Fibrillarin/Nop1p proteins.

Western Blot Dilution: 1:1000

IF Microscopy Dilution: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Détails sur Fibrillarin

Antigène: Fibrillarin (FBL)

Autre désignation: Fibrillarin (FBL Produits)

Synonymes: anticorps FIB, anticorps FLRN, anticorps RNU3IP1, anticorps wu:fb37g12, anticorps zgc:56145, anticorps zgc:77130, anticorps FBL, anticorps AL022665, anticorps CG9888, anticorps Dmel\\CG9888, anticorps Fibri, anticorps GCR-6, anticorps GCR6, anticorps Pen59C5, anticorps fib, anticorps pen59C5, anticorps MGC76139, anticorps fbl, anticorps fibrillarin, anticorps Fibrillarin, anticorps putative fibrillarin, anticorps fibrillarin S homeolog, anticorps FBL, anticorps fbl, anticorps Fbl, anticorps Fib, anticorps fib1, anticorps LMJF_19_0100, anticorps fbl.S

Sujet:

Synonyms: mouse anti-Fibrillarin Antibody, rRNA 2'-O-methyltransferase fibrillarin, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1

Background: Fibrillarin Antibody detects Nop1p which was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5 kDa), is essential for yeast viability, and is localized in the nucleoli. The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin. Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67 % identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34 kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable. Anti-Fibrillarin Antibody is ideal for investigators involved in Cell Proliferation and Cancer Research.

Gene Name: NOP1

ID gène: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process