Lapin anti-Cheval IgG Anticorps (Cy3) - Preadsorbed

N° du produit ABIN6698832

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Reactivité: Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cy3

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Attributs du produit:

Synonyms: Rabbit F(ab')2 Anti-Horse IgG Antibody CY3 Conjugation, Rabbit F(ab')2 Anti-Horse IgG CY3 Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG CY3 Conjugated Antibody

Background: F(ab')2 Anti-Horse IgG Cy3 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Horse IgG whole molecule

Isotype: IgG

Labeling Ratio: 5.1

Information d'application

Indications d'application:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 250 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody